In studying cultures of Spirochæta pallida, for which I am indebted to Dr. H. Noguchi, I have found that the organism stains readily, usually in two or three seconds, with a 10 per cent, mixture of a saturated alcoholic gentian-violet solution in 5 per cent. phenol. The smears may be fixed in the flame, although no fixation is really necessary. The stain is also useful for staining fresh material and for phagocytic experiments. A very thin smear is essential for obtaining a satisfactory stain of fresh material. For phagocytic experiments, the stain is left on about three minutes; the smear should not be fixed by heat; after washing and drying, the leukocytes are stained with Leishman's or some other suitable stain. According to Koessler,1 the stain is satisfactory for staining Spirochæta pallida in experiments with spermatozoa as carriers of infectious agents. After I had found that this was a
TUNNICLIFF R. A SIMPLE STAIN FOR THE SPIROCHÆTA PALLIDA. JAMA. 1912;LVIII(22):1682. doi:10.1001/jama.1912.04260060031011
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