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March 14, 1914

A New Method for Preserving Complement for Making the Wassermann or Noguchi Blood-Test

JAMA. 1914;LXII(11):868. doi:10.1001/jama.1914.02560360048024

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Abstract

Any one familiar with the Wassermann or Noguchi blood-test knows that the complement, or guinea-pig blood-serum, will lose all its complementary power within from forty-eight to seventy-two hours. I fancy that every man doing these tests has at least wondered if one could in any way preserve the guinea-pig serum, and consequently avoid having to collect fresh serum at least twice a week.

I have found by laboratory experiments that a 25 per cent. saline solution will preserve the complement in an ordinary test-tube, if in an ice-box, for two or three weeks, and, if sealed in capillary tubes, for a month or two.

I use a 40 per cent, dilution of guinea-pig blood-serum diluted with a 25 per cent. saline solution. When making the test I use a 0.6 per cent, saline solution instead of the 0.9 per cent. solution as recommended by our leading authorities. When I add

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