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Article
December 2, 1905

A METHOD FOR THE RAPID PREPARATION OF FRESH TISSUES FOR THE MICROSCOPE.

Author Affiliations

Pathologist St. Mary's Hospital. ROCHESTER, MINN.

JAMA. 1905;XLV(23):1737. doi:10.1001/jama.1905.52510230037003c

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Abstract

While engaged in general pathologic work I shared the common distrust of frozen sections of fresh tissues for microscopic diagnosis. On taking charge recently of the laboratories of the Drs. Mayo, surgeons, I carefully tested the various methods hitherto published and found them either too slow for results while the patient waits under the anesthetic or else giving poorly differentiated cell detail. After considerable experimentation the following technic was discovered, and for the last six months it has given uniformly excellent preparations:

  1. Bits of fresh tissue not more than 2×10×10 mm. are frozen in dextrin solution and cut in sections of from 10 to 15 microns thick.

  2. The sections are removed from the knife with the tip of the finger and allowed to thaw thereon.

  3. The sections are unrolled with camel's-hair brushes in 1 per cent. NaCl solution.

  4. The sections are stained from 10 to 20 seconds in neutral Unna's

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