The liver of an ox, just after the animal had been slaughtered, was obtained and passed through a sausage machine, finely dividing the structure and removing a considerable portion of the connective tissue. This finely divided material was stirred up with five times its volume of 1 per cent. sulphuric acid, and the mixture was heated for three hours and thirty minutes in the water bath at a temperature of 81 C. Then it was heated for three hours longer over the direct flame at 100 C. and filtered. The filtrate proved to be a clear, light amber-colored fluid, which was added drop by drop to three volumes of 95 per cent. alcohol, with constant stirring. The precipitate which formed was collected on a hard filter, washed with alcohol, and then dissolved in a minimum amount of water. This solution was again precipitated by adding it drop by drop to
VAUGHAN VC, MUNSON JF, SPENCER FR. THE EXTRACTION OF A TOXIN FROM LIVER CELLS.A PRELIMINARY REPORT. JAMA. 1904;XLII(17):1075. doi:10.1001/jama.1904.92490620017001f
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