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Article
December 1, 1906

DEVIATION OF COMPLEMENT BY SERUM PLUS ANTISERUM AS A TEST FOR BLOOD.

JAMA. 1906;XLVII(22):1832-1833. doi:10.1001/jama.1906.02520220058004
Abstract

Gengou,1 Moreschi2 and others3 have shown that immunization of animals with alien serum or albumins gives rise to antibodies which, on union with constituents (antigens) in the material used for immunization, possess the power to bind complement. This anticomplementary action of serum plus antiserum (antigen plus antibody) is easily demonstrable, because it manifests itself by preventing lysis of red corpuscles by hemolytic serum, normal or immune.

In its simplest form the reaction is carried out as follows: Certain quantities of normal serum, of the corresponding antiserum (both heated to 56 C. for 30 minutes to destroy the native complement) and of normal hemolytic serum are mixed; after one hour there is added the proper amount of 5 per cent, suspension of suitable washed corpuscle; the mixture, together with the necessary controls, are placed in the incubator and the degree of laking determined at the end of two

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