The popular methods for studying the macroscopic and microscopic appearances of the epidermic fungi have been limited to the tube and plate cultures and their modifications on various culture media, and the hanging drop of bouillon ably demonstrates the structural formation of the growth. By the plate culture Sabouraud, Colcott, Fox and others have suggested a classification of the tinea by their clinical appearances and their behavior on solid media. Although they have distinguished certain of the parasitic growths, yet their differentiation from some of the common molds which form cultures not unlike the growth of the trichophyton, can only be corroborated by the microscope. Smears from cultures and cover-glass impressions only suggest stages of a growth and forms of fructification, without a complete history of vegetation. The method which I have entitled cover-glass cuitures consists in culturing the parasite on a cover-glass, using any nutrient media desired, placed
WALLIS JF. COVER-GLASS CULTURES AND THEIR POSSIBILITIES IN STUDYING EPIDERMIC FUNGI. JAMA. 1904;XLIII(8):531–534. doi:10.1001/jama.1904.92500080001b
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