It is a familiar fact that the injection into the animal body of gradually increasing quantities of many substances gives rise to the production of specific antibodies. Among the most carefully studied substances of this class may be mentioned bacterial toxins, living and dead bacteria, red blood corpuscles, blood serum, organ cells, etc. By heating these substances, or by treatment with various chemicals, they are easily deprived of the power of calling forth antibodies. It often is desirable, however, to kill the bacteria that are used in the process of immunization before they are injected, because some living organisms, as, for instance, the tubercle bacilli, are not well borne by any of the animals that are suited for the production of antiserums. In other instances it may be necessary to sterilize the substance before it is used, because of the great difficulty of obtaining it without accidental contamination.
LOEFFLER'S NEW METHOD OF PRODUCING ANTIBODIES.. JAMA. 1905;XLIV(3):221–222. doi:10.1001/jama.1905.02500300053006
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