The results obtained in working teeth for the past few years do not justify a paper on the subject, but these few notes are given to tabulate results—successes and failures—hoping to bring out criticisms on the methods used.
In decalcification the results have been far from satisfactory. In order to protect the dentine and get the pulp and dentine in situ, the slower methods of decalcification are better, but the delay has disadvantages. The acid mixtures penetrate and destroy the staining properties of the tissues and cause distortion. In order to save the pulp, the apical foramina were sealed with collodion or sealing wax; this has not been satisfactory, as the sealing material comes off in spite of careful handling.
Nitric acid in weak solution gives slow decalcification. A 2 per cent. solution with frequent changing every few days, took over one month to give results. Lee says HNO3
ANDERSON M. SOME NOTES CONCERNING PREPARATION OF TEETH FOR MICROSCOPIC STUDY.. JAMA. 1902;XXXIX(3):137–138. doi:10.1001/jama.1902.52480290021001d
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