Repeated filtration of the urine through animal charcoal causes retention by the latter of uric acid, urates, coloring substances, some albumin, but also of certain amounts of dextrose. Powdered animal charcoal, freed from impurities as well as possible by washing and by extraction with hydrogen chlorid, is put to the height of 3 cm. into a filter measuring 5 to 6 cm. in diameter. Twenty to 40 cm. of urine are repeatedly filtered through this layer, until the filtrate is rendered perfectly colorless. As particles of dextrose are retained by the charcoal, this should be washed three times with distilled water, and each of the three washings is examined separately. Fehling's test is then applied to the filtered urine and the washings. The advantage obtained by this procedure is obvious, still decolorized urine may yet possess reducing factors.9 It was found that, notwithstanding the absence of dextrose, the test
STERN H. THE IDENTIFICATION OF DEXTROSE IN HUMAN URINE. JAMA. 1900;XXXV(12):745–751. doi:10.1001/jama.1900.24620380023002e
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