For diagnosis the examination of the fresh unstained blood is usually sufficient, but for the study of the structure of the parasite, dried and stained specimens must be made. The blood is to be spread on the cover-slip in a thin uniform layer, so that the red corpuscles lie separate from each other. To obtain this result the cover-slips must be absolutely free from all foreign material. It is best to immerse them in alcohol and dry them carefully with a soft cloth just before taking the specimens, handling them with forceps rather than with, fingers. A small drop of blood from the cleansed lobe of the ear is allowed to spread between two cover-slips, which are at once slid apart and allowed to dry in the air or by holding high above a flame. Another satisfactory method is to receive the blood from the ear on the edge of
LAZEAR JW. PATHOLOGY OF MALARIAL FEVERS, STRUCTURE OF THE PARASITES AND CHANGES IN TISSUE. JAMA. 1900;XXXV(15):917–920. doi:10.1001/jama.1900.24620410001001
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