The method of research recently introduced by the successful cultivation, by Nocard, of organisms enclosed in collodion sacs and placed in the abdominal cavity of living animals, has been used by Vincent1 in the study of the pathogenic adaptability of saprophytic bacteria. He found that by this method harmless varieties of saprophytes, such as B. megaterium and the potato bacillus, could be changed into virulent organisms capable of producing toxins and causing general infection. As pointed out by Nocard, the walls of the collodion sacs permit of a ready change in the composition of the fluid within them, thus preventing auto-intoxication of the bacteria and at the same time the body fluids enter gradually and furnish sufficient food for the germs, which thus become exposed to the influences of growth under the new conditions.
The bacillus megaterium became virulent after four passages, of six or seven days each, through
THE USE OF INTRA-ABDOMINAL CULTURES IN COLLODION SACS IN THE STUDY OF BACTERIA.. JAMA. 1901;XXXVI(2):111. doi:10.1001/jama.1901.02470020039002
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