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February 4, 1893

A PECULIAR NUCLEAR SAFRANIN REACTION AND ITS RELATION TO THE CARCINOMA COCCIDIA QUESTION.An inaugural thesis read before the Pathological Section of the Chicago Academy of Sciences, January 12, 1893.

JAMA. 1893;XX(5):111-117. doi:10.1001/jama.1893.02420320001001

The employment of a large number of chemical reagents is one of the unfortunate necessities of modern microtomy. No one can become familiar with the long list of substances employed in microscopical research, nor with the bewildering combinations of fixing agents and staining agents, without realizing the possibility of many of these substances being used without a definite knowledge of their effects. The history of microscopic anatomy is full of instances, in which the results of some pioneer investigator have been attacked, on the ground that the results which he obtained were the effects of the reagents employed in the study. In fact, it is even now not uncommon to have the question of the reagent effect brought up, when intricate microscopical studies are being prosecuted. Without doubt, the suspicion that many of the marvellous findings of the cytologists are the products of unknown micro-chemical changes, is still entertained by