Ever since anilin staining methods have been in vogue, there have been demands for a perfect method of fixing sections of tissue on the slide, preparatory to the staining. This demand has arisen from two conditions peculiar to anilin staining processes. The first condition is that only sections of tissue can be stained by any of the processes thus far perfected; that is, it is impossible to stain a piece of tissue in the bulk with the anilin dyes, as can be done with carmine and hematoxylin. The second condition is, that the sections are often so delicate that they are torn or lost in the manipulations required in the process of staining in the watch glass. To meet these difficulties, it has long been the practice of microtomists to secure the sections to the slide; this procedure being termed, "fixing sections on the slide."
Numerous methods of fixing sections
OHLMACHER AP. A COMBINED WATER AND ALBUMEN METHOD OF FIXING PARAFFIN SECTIONS ON THE SLIDE. JAMA. 1893;XX(16):440–442. doi:10.1001/jama.1893.02420430006001d
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