In preparing sections of teeth for the microscope it is necessary to proceed in different ways according to the structures desired to be demonstrated. For convenience the methods may be divided into: 1, ground or hard methods; 2, injection methods, and 3, structural methods; the structural being subdivided into general or those used to demonstrate cellular structures, and special or those demonstrating nervous tissues.
The hard or ground method should be used for studying tooth tissues in situ. The different processes, though differing somewhat in technique, all consist in grinding down sections of teeth thin enough to examine under the microscope and then polishing to a smooth surface and mounting in Canada balsam.
—Fresh tissues only should be used and the method is a rapid one, the sections being ready to mount in thirty minutes. First one side of the tooth and then the other is ground
ANDERSON M. METHODS IN THE PREPARATION OF TEETH. JAMA. 1901;XXXVII(2):90–94. doi:10.1001/jama.1901.62470280014002c
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