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May 12, 1900


JAMA. 1900;XXXIV(19):1202. doi:10.1001/jama.1900.02460190052018

There are times when the determination whether spots on linen are due to seminal fluid or not may be of the highest medicolegal importance, and a method would be welcome that will permit a ready and reliable decision. Florence assumed that the seminal fluid may contain specific alkaloidal substances, and in order to determine this point undertook a study of the effect that the alkaloidal reagents would have on human spermatic fluid. Using a solution of iodin and potassium iodid he was able to obtain a reaction only when the iodin was present in amount sufficient to correspond to a solution of potassium tri-iodid. For this purpose he used 1.65 grams of iodin and 2.51 grams of potassium iodin to 30 grams of water, and added 1 drop of this solution on a glass slide to a watery extract of seminal stain. In the course of a few minutes there