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February 7, 1966

Immunization by Selective Infection With Type 4 Adenovirus Grown in Human Diploid Tissue Culture: II. Specific Protective Effect Against Epidemic Disease

Author Affiliations

From the Naval Medical Field Research Laboratory, Camp Lejeune, NC (Drs. Edmondson and Gundelfinger), and the National Institute of Allergy and Infectious Diseases, Laboratory of Infectious Diseases (Drs. Edmondson, Purcell, Love, Ludwig, and Chanock). Dr. Love is now at the Marine Corps Recruit Depot, Parris Island, SC. Drs. Edmondson and Purcell are Epidemic Intelligence Officers, Communicable Disease Center, assigned to the Laboratory of Infectious Diseases.

JAMA. 1966;195(6):453-459. doi:10.1001/jama.1966.03100060093026

In a previous study (see pp 445-452) the response of adult volunteers to type 4 adenovirus administered in an enteric-coated capsule was evaluated. Infection produced in this manner was limited to the lower intestinal tract and was not associated with any signs or symptoms of illness. Selective intestinal infection with type 4 virus produced moderate levels of neutralizing antibody. In addition, infection did not spread to susceptible contacts despite their prolonged exposure to individuals who shed virus from the intestinal tract. The virus preparation which was studied most extensively was grown in human diploid fibroblast culture (WI 38) and was found to be free of adventitious microbial agents and of oncogenic activity in suckling hamsters. The total experience from the laboratory studies and from the response of the volunteers to enteric infection suggested that administration of type 4 adenovirus in an entericcoated capsule might provide a safe and effective means