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May 16, 1966

Journal of Experimental Medicine, New York

JAMA. 1966;196(7):205-208. doi:10.1001/jama.1966.03100200153091

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In Vitro Differentiation of Mononuclear Phagocytes: Formation of Macrophage Lysosomes—  Z A. Cohn, M. E. Fedorko, and J. G. Hirsch (Rockefeller University, New York)J Exp Med123:757-766 (April 1) 1966A combined morphological, autoradiographic, and cytochemical study at the electron microscope level has been directed towards the formation of electronopaque granules of cultured macrophages. Labeling of the membrane-bound vesicular structures of pinocytic origin was accomplished with colloidal gold. The initial uptake of gold occurred within micropinocytic vesicles. These electron-lucent vesicles subsequently fused with and discharged their contents into larger pinocytic vacuoles. Colloidal gold was homogeneously distributed in the large pinosomes. In contrast, gold was initially deposited in the periphery of preformed dense granules indicating that these structures were also in constant interaction with the external environment. The intracellular flow of leucine-3H-labeled protein was followed by electron microscopic autoradiography. Subsequently, much of the label appeared in the

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