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Sept 14, 1963

Non-Living Spleen Cell Injections: Histocompatibility Barriers Overcome

JAMA. 1963;185(11):28-30. doi:10.1001/jama.1963.03060110008005

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Tolerance to tissue homografts in several strains of mice has been induced by repeated injections of non-living spleen cell material taken from a donor strain of animal.

Dr. Carlos Martinez of the University of Minnesota in Minneapolis told The Journal that "both weak and strong histocompatibility barriers in the mouse have been overcome with repeated injections of this material."

Adult mice were used as spleen cell donors. After the animals were sacrificed, the spleens were removed, freed of the surrounding fat and placed in a tightly fitted glass homogenizer containing lactate-Ringer's saline solution. After reducing the tissue to a homogeneous paste the suspension was subjected to four cycles of rapid freezing and thawing. After the last thawing the spleen suspension was strained through four layers of surgical gauze and checked under the microscope for the presence of intact cells. "In no instance could intact cells be detected in the spleen

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