In previous articles1 it was shown that connective tissue could be kept in vitro for several months in a condition of active life, and that a fragment of heart could pulsate normally for more than one hundred days after its extirpation from the animal; but the quantity of connective tissue maintained in permanent life was very small. It would be important, for the study of many problems, to keep entire organs alive outside of the organism. Therefore I attempted to develop a technic by which a system of organs could be caused to live in vitro.
The method consisted of removing aseptically, en masse, heart, lungs, liver, stomach and part of the intestines, pancreas, adrenals, kidneys and spleen of an animal, a cat generally being used, and preserving these organs in an incubator at the temperature of 38 C. (100.4 F.), while the lungs were being artificially ventilated.
CARREL A. VISCERAL ORGANISMS. JAMA. 1912;LIX(24):2105–2106. doi:10.1001/jama.1912.04270120090001
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