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August 11, 1962

Clinical Science

JAMA. 1962;181(6):476-484. doi:10.1001/jama.1962.03050320014003

Studies on the Rheumatoid and Related Serum Factors 

II. Relation of Anti-Human and Anti-Rabbit Gamma Globulin Factors in Rheumatoid Arthritis Serums  Felix Milgrom, M.D., Ernest Witebsky, M.D., Robert Goldstein, and Ulana Loza, M.D., BuffaloTHE SEROLOGY of rheumatoid arthritis is attracting increasing attention. Little doubt remains that the laboratory tests based on detecting the rheumatoid factor constitute helpful diagnostic tools. Clinical evaluation of these tests, as well as serological and physicochemical properties of the rheumatoid factor, have been subjects of several reviews.1-5 Summarizing the data available, one may say that this factor has serological properties of an antibody against gamma globulin of human and rabbit origin. The question arose as to whether the same molecule of the rheumatoid factor could combine with human and rabbit gamma globulin, or whether there exist different molecules for each of these gamma globulins.Lospalluto and Ziff6 separated the rheumatoid factor by cation

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Grubb, R.:  Gm Groups and Their Relation to Rheumatoid Arthritis Serology ,  Arthritis Rheum 4:195-202 ( (April) ) 1961.Crossref
Milgrom F.; and Witebsky, E.:  Rheumatoid Factor and Its Possible Pathogenic Role ,  Second International Symposium of Immunopathology , Basel: Benno Schwabe and Co., to be published.
Lospalluto, J., and Ziff, M.:  Chromatographic Studies of Rheumatoid Factor ,  J Exp Med 110:169-186 ( (Aug.) ) 1959.Crossref
Heimer, R., and Schwartz, E. R.:  Isolation of Rheumatoid Factors ,  Arthritis Rheum 4:153-160 ( (April) ) 1961.Crossref
Lawler, S. D.:  Genetical Study of Gm Groups in Human Serum ,  Immunology 3:90-94 ( (Jan.) ) 1960.
Topley, W. W. C.; Wilson, J.; and Duncan, J. T.:  Mode of Formation of Aggregates in Bacterial Agglutination ,  Brit J Exp Path 16:116-120 ( (April) ) 1935.
Finland, M., and Curnen, E. C.:  Agglutinins for Human Erythrocytes in Type XIV Anti-Pneumococcic Horse Serums ,  Science 87:417-418 ( (May) ) 1938.Crossref
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Marrack, J. R.: Chemistry of Antigens and Antibodies, London: His Majesty's Stationery Office, 1934.
Coombs, R. R. A.:  Mixed Agglutination Reaction in Study of Normal and Malignant Cells ,  Cancer Res 21:1198-1202 ( (Oct.) ) 1961.
Cohen E., et al.:  Use of Alligator Erythrocytes for Demonstrating Agglutination Activating Factor in Rheumatoid Arthritis ,  Amer J Clin Path 30:32-34 ( (July) ) 1958.
Milgrom, F., et al.:  Red Blood Cells Sensitized by Incomplete Rabbit Antibodies as Reagent for Detecting Rheumatoid Factor ,  Acta Rheum Scand , to be published.
Waller, M. V., and Vaughan, J. H.:  Use of Anti-Rh Sera for Demonstrating Agglutination Activating Factor in Rheumatoid Arthritis ,  Proc Soc Exp Biol Med 92:198-200 ( (May) ) 1956.Crossref
Grubb, R.:  Agglutination of Erythrocytes Coated with "Incomplete" Anti-Rh by Certain Rheumatoid Arthritic Sera and Some Other Sera: Existence of Human Serum Groups ,  Acta Path Microbiol Scand 39:195-197 (Fasc. (3) ) 1956.Crossref
Milgrom, F.; Dubiski, S.; and Wozniczko, G.:  Human Sera with "Anti-Antibody"  Vox Sang 1:172-183 (No. (3) ) 1956.Crossref
Kellner, A., and Hedal, E.:  Experimental Erythroblastosis Fetalis in Rabbits: I. Characterization of Pair of Allelic Blood Group Factors and Their Specific Immune Isoantibodies ,  J Exp Med 97:33-49 ( (Jan.) ) 1953.Crossref
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Franklin, E. C., et al.:  Unusual Protein Component of High Molecular Weight in Serum of Certain Patients with Rheumatoid Arthritis ,  J Exp Med 105:425-438 ( (May) ) 1957.Crossref
Hektoen, L., and Boor, A. K.:  Simultaneous Multiple Immunization ,  J Infect Dis 48:588-594 ( (June) ) 1931.Crossref
Dean, H. R.; Taylor, G. L.; and Adair, M. E.:  Precipitation Reaction: Experiments with Antiserum Containing Two Antibodies ,  J Hyg 35:60-74 ( (Fed.) ) 1935.Crossref
Heidelberger, M., and Kabat, E. A.:  Quantitative Studies on Antibody Purification: II. Dissociation of Antibody from Pneumococcus Specific Precipitates and Specifically Agglutinated Pneumococci ,  J Exp Med 67:181-199 ( (Feb) ) 1938.Crossref
Milgrom, F.:  Badania nad budowa przeciwcial (Researches on the Structure of Antibodies) , edited by Panstwowy Zaklad Wydawnictw Lekarskich, Warszawa, 1950.
Dodd, B. E.:  Linked Anti-A and Anti-B Antibodies from Group O Sera ,  Brit J. Exp Path 33:1-18 ( (Feb.) ) 1952.
Milgrom, F.:  Recherches sur la structure des isoanticorps groupaux ,  Rev Immun (Par) 16:86-109 (No. (3) ) 1952.
Bird, G. W. G.:  Observations on Haemagglutin "Linkage" in Relation to Iso-Agglutinins and Auto-Agglutinins ,  Brit J Exp Path 34:131-137 ( (April) ) 1953.
Milgrom, F., and Swierczynska, Z.:  Studies on Specificity of Natural Antibacterial Antibodies ,  Schweiz Z Allg Path 19:189-204 (No. (2) ) 1956.
Milgrom, F.; Orellana, C.; and Layrisse, M.:  Recovery of Blood Group Antibodies from Erythrocyte Powder ,  Blood 14:913-919 ( (Aug.) ) 1959.
Milgrom, F., and Witebsky, E.:  Studies on Rheumatoid and Related Serum Factors: I. Autoimmunization of Rabbits with Gamma Globulin ,  JAMA 174:56-63 ( (Sept.) ) 1960.Crossref
Milgrom, F., and Witebsky, E.:  Autoantibodies and Autoimmune Diseases ,  JAMA , to be published.