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November 13, 1967

Endocrine Colpocytology and Cytodiagnosis of Ruptured Fetal Membranes

Author Affiliations

From the Department of Obstetrics-Gynecology, University of Miami (Fla) School of Medicine.

JAMA. 1967;202(7):640-644. doi:10.1001/jama.1967.03130200126022

During the past ten years at the University of Miami, we have studied the diagnostic value of a rapid staining technique in evaluating vaginal and cervical smears taken from obstetric and gynecologic patients. For obstetric patients, we examined smears for the presence of vernix caseosa or fetal squamous cells. The absence of these cells is a precise indicator of the integrity of fetal membranes in questionable instances of premature rupture of the amnion. For gynecologic patients, our objective was to obtain colpocytologic evidence of estrogen and progesterone production. This is a report of our experiences with vaginal cytology in the daily management of patients.

Staining Technique and Results 

Cytodiagnosis of Ruptured Membranes.—  To test for ruptured membranes, cellular samples are obtained by inserting the sterile, gloved index finger in the external cervical os and the middle finger in the posterior fornix. Spreads are made on two clean glass slides and

Wargo, J.D., and Hopman, B.C.:  Cytodiagnosis of Ruptured Amnion ,  Bull Univ Miami Sch Med 11:81-85 ( (Sept) ) 1957.
Averette, H.E.; Hopman, B.C.; and Ferguson, J.H.:  Cytodiagnosis of Ruptured Fetal Membranes: Further Experiences ,  Amer J Obstet Gynec 87:226-230 ( (Sept 15) ) 1963.
Brosens, I., and Gordon, H.:  The Cytological Diagnosis of Ruptured Membranes Using Nile Blue Sulfate Staining ,  J Obstet Gynec Brit Comm 72:342 ( (June) ) 1965.Crossref
Shorr, E.:  New Technic for Staining Vaginal Smears: Single Differential Stain ,  Science 94:545 ( (Dec 5) ) 1941.Crossref
Mack, H.C.:  Vaginal Smear Technic ,  J Clin Endocr 3:169 ( (March) ) 1943.Crossref