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To the Editor:
—I wish to call attention to a method of differentiating the various modifications of the Romanowsky stains, by means of which not only may very beautiful preparations be obtained, but far more accurate knowledge gained of the structure of the nucleus of protozoa. I have been using the method for several months in the study of the common if not the sole pathogenic entameba of this region—Entamæba tetragena, Smears are stained first with Leishman's, Wright's or Hastings' stain, and if, on inspection, a smear contains a sufficient number of entamebas for further study, it is then stained by Giemsa's stain until the film has a purple cast. It is then plunged into 60 per cent. alcohol, to which 10 to 20 drops of aqua ammoniæ have been added. The slide is kept in motion and examined from time to time, with either the highdry or water
Darling ST. Romanowsky Stain for Entamebas. JAMA. 1912;LIX(4):292. doi:10.1001/jama.1912.04270070291024
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