[Skip to Content]
Sign In
Individual Sign In
Create an Account
Institutional Sign In
OpenAthens Shibboleth
Purchase Options:
[Skip to Content Landing]
Article
February 20, 1967

Clinical Evaluation of Cryoprecipitated Factor VIII

Author Affiliations

From the Department of Pathology (Drs. Simson and Oberman), and the Simpson Memorial Institute (Dr. Penner), the University of Michigan, Ann Arbor.

JAMA. 1967;199(8):554-558. doi:10.1001/jama.1967.03120080088015
Abstract

Although there is increasing reason to believe that the pathogenesis of classical hemophilia involves much more than deficiency of a plasma clotting factor,1 treatment of bleeding episodes remains firmly based upon the infusion of factor VIII.2 The rationale of plasma transfusion therapy has been well defined3 and assay procedures4,5 have been developed which permit an accurate diagnosis of hemophilia (hemophilia A), and, in addition, enable quantitative assessment of therapy. Nevertheless, treatment of this disorder still is beset with difficulties.

Large and frequent transfusions of fresh, freshfrozen, or reconstituted freeze-dried plasma are required to achieve and maintain hemostatic levels of factor VIII. Daily transfusions approximating one third of the patient's circulating plasma volume are required over a period of weeks to control hemorrhage following surgical procedures. Such massive plasma transfusions present the concomitant risk of circulatory overload. Moreover, prolonged plasma therapy limits the mobility of the patient

References
1.
Mammen, E.:  Investigation on the Nature of Hemophilia A ,  Thromb Diath Haemorrh 9:30-52, 1963.
2.
Simson, L.R.; Oberman, H.A.; and Penner, J.A.:  Preparation and Clinical Evaluation of Cryo-Factor VIII , abstracted,  Clin Res 14:327 ( (April) ) 1966.
3.
Brinkhous, K.M., et al:  Physiological Basis of Transfusion Therapy in Hemophilia ,  Arch Path 61:6-10 ( (Jan) ) 1956.
4.
Langdell, R.D.; Wagner, R.H.; and Brinkhous, K.M.:  Effect of Anti-Hemophilic Factor on One-Stage Clotting Tests ,  J Lab Clin Med 41:637-647 ( (April) ) 1953.
5.
Penner, J.A.:  Measurement of Antihemophilic Factor Activity in Plasma ,  J Lab Clin Med 67:1025-1035 ( (June) ) 1966.
6.
Cohn, E.J.:  Preparation and Properties of Serum and Plasma Proteins: IV. System for Separation Into Fractions of Protein and Lipoprotein Components of Biological Fluids ,  J Amer Chem Soc 6:459-475 ( (March) ) 1946.Crossref
7.
Minot. G.R., et al:  Coagulation Defect in Hemophilia: Effect, in Hemophilia, of Parenteral Administration of Fraction of Plasma Globulins Rich In Fibrinogen ,  J Clin Invest 24:704-707 ( (Sept) ) 1945.Crossref
8.
Webster, W.P., et al:  Clinical Use of a New Glycine-Precipitated Antihemophilic Fraction ,  Amer J Med Sci 250:643-650 ( (Dec) ) 1965.Crossref
9.
Pool, J.G., and Robinson, J.:  Observations on Plasma Banking and Transfusion Procedures for Hemophilic Patients Using Quantitative Assay for Antihemophilic Globulin ,  Brit J Haemat 5:24-30 ( (Jan) ) 1959.Crossref
10.
Brinkhous, K.M.:  Plasma Antihemophilic Factor: Biological and Clinical Aspects ,  Sang 25:738-741, 1954.
11.
Ware, A.G.; Guest, M.M.; and Seegers, W.H.:  Fibrinogen: With Special Reference to its Preparation and Certain Properties of the Product ,  Arch Biochem 13:231-236 ( (May) ) 1947.
12.
Pool, J.G., and Shannon, A.E.:  Simple Production of High Potency Antihemophilic Globulin (AHG) Concentrates in a Closed Bag System , abstracted,  Fed Proc 24:512 ( (March) -April) 1965.
13.
Djerassi, I., et al:  Clinical Use of Cold Precipitated Antihemophilic Globulin (Factor VIII, CPAG) ,  Transfusion 5:533-538 ( (Nov) -Dec) 1965.Crossref
14.
Simson, L.R., et al:  A Method for Preparing Plasma Factor VIII (Anti-Hemophilic Globulin) Concentrate ,  Amer J Clin Path 45:373-376 ( (April) ) 1966.
15.
Britten, A., and Grove-Rasmussen, M.:  Stability of Factor VIII in the Frozen State ,  Transfusion 6:230-233 ( (May) -June) 1966.Crossref
16.
Barrow, E.M.; Bullock, W.R.; and Graham, J.B.:  A Study of the Carrier State for Plasma Thromboplastin Component (PTC, Christmas Factor) Deficiency, Utilizing a New Assay Procedure ,  J Lab Clin Med 55:936-945 ( (June) ) 1960.
17.
Proctor, R.R., and Rapaport, S.I.:  The Partial Thromboplastin Time With Kaolin: A Simple Screening Test For First Stage Plasma Clotting Factor Deficiencies ,  Amer J Clin Path 36:212-219 ( (Sept) ) 1961.
18.
Ware, A.G., and Seegers, W.H.:  Two-Stage Procedure For The Quantitative Determination of Prothrombin Concentration ,  Amer J Clin Path 19:471-482 ( (May) ) 1949.
19.
Pool, J.G., and Shannon, A.E.:  Production of High-Potency Concentrates of Antihemophilic Globulin in a Closed-Bag System, Assay In Vitro and In Vivo ,  New Eng J Med 273:1443-1447 ( (Dec) ) 1965.Crossref
×