What is the incidence and significance of cardiac injury in patients with COVID-19?
In this cohort study of 416 consecutive patients with confirmed COVID-19, cardiac injury occurred in 19.7% of patients during hospitalization, and it was one independent risk factor for in-hospital mortality.
Cardiac injury is a common condition among patients hospitalized with COVID-19, and it is associated with higher risk of in-hospital mortality.
Coronavirus disease 2019 (COVID-19) has resulted in considerable morbidity and mortality worldwide since December 2019. However, information on cardiac injury in patients affected by COVID-19 is limited.
To explore the association between cardiac injury and mortality in patients with COVID-19.
Design, Setting, and Participants
This cohort study was conducted from January 20, 2020, to February 10, 2020, in a single center at Renmin Hospital of Wuhan University, Wuhan, China; the final date of follow-up was February 15, 2020. All consecutive inpatients with laboratory-confirmed COVID-19 were included in this study.
Main Outcomes and Measures
Clinical laboratory, radiological, and treatment data were collected and analyzed. Outcomes of patients with and without cardiac injury were compared. The association between cardiac injury and mortality was analyzed.
A total of 416 hospitalized patients with COVID-19 were included in the final analysis; the median age was 64 years (range, 21-95 years), and 211 (50.7%) were female. Common symptoms included fever (334 patients [80.3%]), cough (144 [34.6%]), and shortness of breath (117 [28.1%]). A total of 82 patients (19.7%) had cardiac injury, and compared with patients without cardiac injury, these patients were older (median [range] age, 74 [34-95] vs 60 [21-90] years; P < .001); had more comorbidities (eg, hypertension in 49 of 82 [59.8%] vs 78 of 334 [23.4%]; P < .001); had higher leukocyte counts (median [interquartile range (IQR)], 9400 [6900-13 800] vs 5500 [4200-7400] cells/μL) and levels of C-reactive protein (median [IQR], 10.2 [6.4-17.0] vs 3.7 [1.0-7.3] mg/dL), procalcitonin (median [IQR], 0.27 [0.10-1.22] vs 0.06 [0.03-0.10] ng/mL), creatinine kinase–myocardial band (median [IQR], 3.2 [1.8-6.2] vs 0.9 [0.6-1.3] ng/mL), myohemoglobin (median [IQR], 128 [68-305] vs 39 [27-65] μg/L), high-sensitivity troponin I (median [IQR], 0.19 [0.08-1.12] vs <0.006 [<0.006-0.009] μg/L), N-terminal pro-B-type natriuretic peptide (median [IQR], 1689 [698-3327] vs 139 [51-335] pg/mL), aspartate aminotransferase (median [IQR], 40 [27-60] vs 29 [21-40] U/L), and creatinine (median [IQR], 1.15 [0.72-1.92] vs 0.64 [0.54-0.78] mg/dL); and had a higher proportion of multiple mottling and ground-glass opacity in radiographic findings (53 of 82 patients [64.6%] vs 15 of 334 patients [4.5%]). Greater proportions of patients with cardiac injury required noninvasive mechanical ventilation (38 of 82 [46.3%] vs 13 of 334 [3.9%]; P < .001) or invasive mechanical ventilation (18 of 82 [22.0%] vs 14 of 334 [4.2%]; P < .001) than those without cardiac injury. Complications were more common in patients with cardiac injury than those without cardiac injury and included acute respiratory distress syndrome (48 of 82 [58.5%] vs 49 of 334 [14.7%]; P < .001), acute kidney injury (7 of 82 [8.5%] vs 1 of 334 [0.3%]; P < .001), electrolyte disturbances (13 of 82 [15.9%] vs 17 of 334 [5.1%]; P = .003), hypoproteinemia (11 of 82 [13.4%] vs 16 of 334 [4.8%]; P = .01), and coagulation disorders (6 of 82 [7.3%] vs 6 of 334 [1.8%]; P = .02). Patients with cardiac injury had higher mortality than those without cardiac injury (42 of 82 [51.2%] vs 15 of 334 [4.5%]; P < .001). In a Cox regression model, patients with vs those without cardiac injury were at a higher risk of death, both during the time from symptom onset (hazard ratio, 4.26 [95% CI, 1.92-9.49]) and from admission to end point (hazard ratio, 3.41 [95% CI, 1.62-7.16]).
Conclusions and Relevance
Cardiac injury is a common condition among hospitalized patients with COVID-19 in Wuhan, China, and it is associated with higher risk of in-hospital mortality.
Since December 2019, coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in considerable morbidity and mortality in more than 30 countries worldwide. Recently, COVID-19–associated clusters of severe respiratory illness have been independently associated with risk of mortality, and mounting evidence substantiates the presence of cardiac injury in patients with COVID-19.1,2 Although a recent study reported that 12% of patients had COVID-19–associated acute cardiac injury,1 manifesting as an ejection fraction decline and troponin I elevation, and the American College of Cardiology clinical bulletin has highlighted the cardiac implications of COVID-19,3 the association between COVID-19–associated cardiac injury and risk of mortality remains unclear. The present study therefore retrospectively analyzed data from a single center in Wuhan, China, to examine the potential association between cardiac injury and mortality among patients with COVID-19.
Consecutive patients admitted to Renmin Hospital of Wuhan University with laboratory-confirmed COVID-19 were included in this retrospective cohort study, which was conducted from January 20, 2020, to February 10, 2020. Renmin Hospital of Wuhan University, located in Wuhan, Hubei Province, China, was assigned responsibility for the treatment of patients with severe COVID-19 by the Wuhan government. The patients with COVID-19 enrolled in this study were diagnosed according to World Health Organization interim guidance.4 The cases without cardiac biomarkers, including values of high-sensitivity troponin I (hs-TNI) and creatinine kinase–myocardial band (CK-MB), were excluded.
This study was approved by the National Health Commission of China and the institutional review board at Renmin Hospital of Wuhan University (Wuhan, China). Written informed consent was waived by the ethics commission of the designated hospital for patients with emerging infectious diseases.
The demographic characteristics (age and sex), clinical data (symptoms, comorbidities, laboratory findings, treatments, complications, and outcomes), laboratory findings, and results of cardiac examinations (cardiac biomarkers and electrocardiography) for participants during hospitalization were collected from electronic medical records by 2 investigators (S.S. and B.S.). Cardiac biomarkers measured on admission were collected, including hs-TNI, CK-MB, and myohemoglobin. The radiologic assessments included chest radiography or computed tomography. All data were independently reviewed and entered into the computer database by 2 analysts (T.L. and Y.C.). Patients were categorized according to the presence or absence of cardiac injury. Cardiac injury was defined as blood levels of cardiac biomarkers (hs-TNI) above the 99th-percentile upper reference limit, regardless of new abnormalities in electrocardiography and echocardiography. Acute respiratory distress syndrome (ARDS) was defined according to the Berlin definition.5 Acute kidney injury was identified according to the Kidney Disease: Improving Global Outcomes definition.6 The clinical outcomes (ie, discharges, mortality, and length of stay) were monitored up to February 15, 2020, the final date of follow-up.
To confirm COVID-19, the Viral Nucleic Acid Kit (Health) was used to extract nucleic acids from clinical samples according to the kit instructions. A 2019-nCoV detection kit (Bioperfectus) was used to detect the ORF1ab gene (nCovORF1ab) and the N gene (nCoV-NP) according to the manufacturer’s instructions, using real-time reverse transcriptase–polymerase chain reaction.7 An infection was considered laboratory-confirmed if the nCovORF1ab and nCoV-NP tests both showed positive results.
Descriptive statistics were obtained for all study variables. All categorical variables were compared for the study outcome by using the Fisher exact test or χ2 test, and continuous variables were compared using the t test or the Mann-Whitney U test, as appropriate. Continuous data are expressed as mean (SD) or median (interquartile range [IQR]) values. Categorical data are expressed as proportions. Survival curves were plotted using the Kaplan-Meier method and compared between patients with vs without cardiac injury using the log-rank test. Multivariate Cox regression models were used to determine the independent risk factors for death during hospitalization. Data were analyzed using SPSS version 25.0 (IBM). Statistical charts were generated using Excel 2016 (Microsoft) or Prism 5 (Graphpad). For all the statistical analyses, P < .05 was considered significant.
Figure 1 shows a flowchart for patient recruitment. Briefly, of all 1004 patients in the medical record system who were screened initially from January 20, 2020, to February 10, 2020, 218 patients whose cases were not confirmed, 141 patients without available medical information and duplicated records, and 229 patients with missing core results of laboratory examination (hs-TNI and CK-MB) were excluded. The median age of these 229 patients was 45 years (range, 22-90 years), and 130 (56.8%) were female; the details of baseline characteristics were presented in the eTable in the Supplement.
Finally, the study population included 416 patients hospitalized with confirmed COVID-19: 82 patients (19.7%) with cardiac injury and 334 patients (80.3%) without cardiac injury. The median age was 64 years (range, 21-95 years), and 211 (50.7%) were female. Among these patients, fever (334 patients [80.3%]) was the most common symptom. Cough, shortness of breath, fatigue, sputum production, and muscle ache were present in 144 patients (34.6%), 117 patients (28.1%), 55 patients (13.2%), 23 patients (5.5%), and 19 patients (4.6%), respectively. Diarrhea (16 patients [3.8%]), chest pain (14 patients [3.4%]), sore throat (12 patients [2.9%]), rhinorrhea (10 patients [2.4%]), and headache (9 patients [2.2%]) were rare. Hypertension (127 patients [30.5%]) and diabetes (60 patients [14.4%]) were the most common coexisting conditions. Of these 416 patients, 44 (10.6%) and 22 (5.3%) had coronary heart disease and cerebrovascular disease, respectively. The proportion of chronic heart failure, chronic renal failure, chronic obstructive pulmonary disease, cancer, pregnancy, and hepatitis B infection was 4.1% (17 patients), 3.4% (14 patients), 2.9% (12 patients), 2.2% (9 patients), 1.7% (7 patients), and 1.0% (4 patients), respectively.
Compared with patients without cardiac injury, patients with cardiac injury were older (median [range] age, 74 [34-95] years vs 60 [21-90] years; P < .001), and more likely to have chest pain (11 of 82 patients [13.4%] vs 3 of 334 patients [0.9%]; P < .001). Moreover, comorbidities, including hypertension (49 [59.8%] vs 78 [23.4%]), diabetes (20 [24.4%] vs 40 [12.0%]), coronary heart disease (24 [29.3%] vs 20 [6.0%]), cerebrovascular disease (13 [15.9%] vs 9 [2.7%]), chronic heart failure (12 [14.6%] vs 5 [1.5%]), chronic obstructive pulmonary disease (6 [7.3%] vs 6 [1.8%]), and cancer (7 [8.5%] vs 2 [0.6%]), were present more often among patients with cardiac injury (all P < .001) (Table 1).
Laboratory and Radiographic Findings
The laboratory and radiologic findings are shown in Table 1. In the overall study population of 416 patients, median (IQR) levels of C-reactive protein (4.5 [1.4-8.5] mg/dL; to convert to milligrams per liter, multiply by 10) and procalcitonin (0.07 [0.04-0.15] ng/L) were elevated, while the median values of other laboratory indicators were within the normal range, such as counts of leukocytes, lymphocytes, platelets, erythrocytes; hemoglobin level; cardiac indicators; alanine aminotransferase level; aspartate aminotransferase level; creatinine concentration; and electrolyte levels. The proportion of patietns with bilateral pneumonia was 74.8% (311 patients) according to chest radiography and computed tomography findings, and 68 patients (16.3%) had multiple mottling and ground-glass opacity. Distribution of hs-TNI based on detection time from hospitalization (median [IQR], 2 [1-15] days) is shown in eFigure 1 in the Supplement. The duration of hospitalization before testing was longer in patients with cardiac injury than those without cardiac injury (median [range] time, 3 [1-15] days vs 2 [1-8] days; P < .001).
In terms of laboratory findings, patients with cardiac injury compared with patients without cardiac injury showed higher median leukocyte count (median [IQR], 9400 [6900-13 800] cells/μL vs 5500 [4200-7400] cells/μL), and levels of C-reactive protein (median [IQR], 10.2 [6.4-17.0] mg/dL vs 3.7 [1.0-7.3] mg/dL), procalcitonin (median [IQR], 0.27 [0.10-1.22] ng/mL vs 0.06 [0.03-0.10] ng/mL), CK-MB (median [IQR], 3.2 [1.8-6.2] ng/mL vs 0.9 [0.6-1.3] ng/mL), myohemoglobin (median [IQR], 128 [68-305] μg/L vs 39 [27-65] μg/L), hs-TNI (median [IQR], 0.19 [0.08-1.12] μg/L vs <0.006 [<0.006-0.009] μg/L), N-terminal pro-B-type natriuretic peptide (NT-proBNP) (median [IQR], 1689 [698-3327] pg/mL vs 139 [51-335] pg/mL), aspartate aminotransferase (median [IQR], 40 [27-60] U/L vs 29 [21-40] U/L), and creatinine (median [IQR], 1.15 [0.72-1.92] mg/dL vs 0.64 [0.54-0.78] mg/dL) during hospitalization, but a lower median lymphocyte count (median [IQR], 600 [400-900] cells/μL vs 1000 [800-1400] cells/μL), platelet count (median [IQR], 172 [111-215] cells × 103/μL vs 216 [165-273] cells × 103/μL), and albumin level (median [IQR], 3.2 [2.9-3.4] g/dL vs 3.7 [3.3-3.9] g/dL), with significant differences in each case (all P < .001; Table 1; eFigure 1 in the Supplement). In terms of radiologic findings, bilateral pneumonia (75 of 82 patients [91.5%] vs 236 of 334 patients [70.7%]) and multiple mottling and ground-glass opacity (53 [64.6%] vs 15 [4.5%]) were more prevalent in patients with than those without cardiac injury (both P < .001, Table 1).
Of patients with cardiac injury, only 22 (26.8%) underwent examination of electrocardiogram (ECG) after admission, and 14 of 22 ECGs (63.6%) were performed during the periods of elevation of cardiac biomarkers. All 14 ECGs were abnormal, with findings compatible with myocardial ischemia, such T-wave depression and inversion, ST-segment depression, and Q waves. The ECG changes in 3 patients with representative cardiac injury are shown in eFigure 2 in the Supplement.
Treatment, Complications, and Clinical Outcome
The median time from symptom onset to admission was 10 (IQR, 1-30) days and similar between the 2 groups (P = .27; Table 2). A total of 399 patients (95.9%) were treated with oxygen, and the percentages of use of oxygen inhalation, noninvasive ventilation, and invasive mechanical ventilation were 76.0% (316 patients), 12.3% (51 patients), and 7.7% (32 patients), respectively. The proportion of antiviral therapy use was the highest (403 [96.9%]), followed by glucocorticoids (304 [73.1%]), intravenous immunoglobulin therapy (259 [62.3%]), and antibiotic therapy (235 [56.5%]). Only 2 patients (0.5%) among all participants were given continuous kidney therapy. Overall, 97 patients (23.3%) had ARDS, and 8 patients (1.9%) had acute kidney injury during hospitalization; other common complications included electrolyte disturbance (30 patients [7.2%]), hypoproteinemia (27 [6.5%]), anemia (13 [3.1%]), and coagulation disorders (12 [2.9%]). During follow-up, a total of 57 patients (13.7%) died, 40 patients (9.6%) were discharged, and the rest (319 [76.7%]) remained hospitalized. Compared with those without cardiac injury, patients with cardiac injury required more noninvasive ventilation (38 [46.3%] vs 13 [3.9%]; P < .001) and invasive mechanical ventilation (18 [22.0%] vs 14 [4.2%]; P < .001) (Table 2). The use of antibiotic treatment (68 [82.9%] vs 167 [50.0%]), glucocorticoids (72 [87.8%] vs 232 [69.5%]), and intravenous immunoglobulin treatment (68 [82.9%] vs 191 [57.2%]) was also significant higher in patients with cardiac injury than in those without cardiac injury (all P < .001; Table 2). In addition to anemia, other complications were more common among patients with cardiac injury than those without cardiac injury; these included ARDS (48 [58.5%] vs 49 [14.7%]; P < .001), acute kidney injury (7 [8.5%] vs 1 [0.3%]; P < .001), electrolyte disturbances (13 [15.9%] vs 17 [5.1%]; P = .003), hypoproteinemia (11 [13.4%] vs 16 [4.8%]; P = .01), and coagulation disorders (6 [7.3%] vs 6 [1.8%]; P = .02) (Table 2).
Cardiac Injury and Mortality
Patients with cardiac injury vs those without cardiac injury had shorter durations from symptom onset to follow-up (mean, 15.6 [range, 1-37] days vs 16.9 [range, 3-37] days; P = .001) and admission to follow-up (6.3 [range, 1-16] days vs 7.8 [range, 1-23] days; P = .039). The mortality rate was higher among patients with vs without cardiac injury (42 [51.2%] vs 15 [4.5%]; P < .001) as shown in Table 2 and the Kaplan-Meier survival curves in Figure 2. The mortality rate increased in association with the magnitude of the reference value of hs-TNI (eFigure 3 in the Supplement). After adjusting for age, preexisting cardiovascular diseases (hypertension, coronary heart disease, and chronic heart failure), cerebrovascular diseases, diabetes mellitus, chronic obstructive pulmonary disease, renal failure, cancer, ARDS, creatinine levels greater than 133 μmol/L, and NT-proBNP levels greater than 900 pg/mL, the multivariable adjusted Cox proportional hazard regression model showed a significantly higher risk of death in patients with cardiac injury than in those without cardiac injury, either during time from symptom onset (hazard ratio [HR], 4.26 [95% CI, 1.92-9.49]) or time from admission to study end point (HR, 3.41 [95% CI, 1.62-7.16]) (Table 3). Under this hazard regression model, ARDS was another independent risk factor for mortality with COVID-19, with a high HR (7.89 [95% CI, 3.73-16.66]) in model 1 and an HR of 7.11 (95% CI, 3.31-15.25) in model 2 (Table 3).
The present study demonstrates the statistically significant association between cardiac injury and mortality in patients with COVID-19. Cardiac injury, as a common complication (19.7%), was associated with an unexpected high risk of mortality during hospitalization.
As of March 12, 2020, there have been a total of more than 130 000 laboratory-confirmed cases of COVID-19 globally, including more than 80 000 within mainland China. Because of its high infectivity, this virus has managed to supersede severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) in death toll. Severe respiratory distress is usually considered the main cause of coronavirus-induced death. According to a recent study of the largest clinical sample in China,8 severe pneumonia was independently associated with admission to an intensive care unit, mechanical ventilation, or death. It is notable that a recent report on 138 patients hospitalized with COVID-19 found that 7.2% of patients developed acute cardiac injury, and patients who received care in the intensive care unit were more likely to have cardiac injury (22.2%) than non-ICU patients.2 This observation suggests that cardiac injury is possibly associated with the clinical outcomes of COVID-19. Consistently, our study also found 19.7% of patients with cardiac injury and first demonstrated that cardiac injury was independently associated with an increased risk of mortality in patients with COVID-19. Compared with patients without cardiac injury, patients with cardiac injury presented with more severe acute illness, manifested by abnormal laboratory and radiographic findings, such as higher levels of C-reactive protein, NT-proBNP, and creatinine levels; more multiple mottling and ground-glass opacity; and a greater proportion requiring noninvasive or invasive ventilation.
In a study of cardiovascular complications of SARS in 121 patients,9 hypertension occurred in 61 patients (50.4%) in the hospital. Of these patients, 71.9% developed persistent tachycardia, including 40% with continued tachycardia during outpatient follow-up. Although tachycardic cardiovascular complications were common in patients with SARS, they were usually self-limiting and not associated with risk of death. In contrast with that from SARS, more than half of the patients with cardiac injury experienced in-hospital death in this study, indicating that COVID-19–induced cardiac injury is associated with major adverse clinical outcomes. However, the mechanism of cardiac injury among these patients with COVID-19 remains uncertain.
Evidence from a case report showed that MERS-CoV causes acute myocarditis, manifested as myocardial edema and acute myocardial injury of the apical and lateral walls of the left ventricle.10 This regional myocardial injury may result from direct viral myocardial infection. On the basis of recent studies, angiotensin-converting enzyme 2 (ACE2) is a human cell receptor with a strong binding affinity to the Spike protein of SARS-CoV-2, and ACE2 is also highly expressed in heart.11,12 Thus, it is rational to hypothesize that COVID-19–induced cardiac injury might be mediated by ACE2. However, a recent pathological study found scarce interstitial mononuclear inflammatory infiltrates in heart tissue without substantial myocardial damage in a patient with COVID-19,13 suggesting that COVID-19 might not directly impair the heart. The present study lacks evidence from magnetic resonance imaging or echocardiography to determine the features of myocardial injury. On the basis of the present results of hs-TNI and ECG findings in a subset of patients, we can only estimate the severity of cardiac injury. Thus, because of the current limited evidence, the question of whether the SARS-CoV-2 virus can directly injure the heart requires further demonstration.
In contrast, a previous study found that reversible, subclinical diastolic left ventricular impairment appears to be common in acute SARS infection, even among those without underlying cardiac disease,14 suggesting that left ventricular dysfunction in the acute phase might be attributable to the cytokine storm syndrome. This is a serious life-threatening disease with clinical features of systemic inflammation, methemoglobinemia, hemodynamic instability, and multiple organ failure.15,16 The hallmark of cytokine storm syndrome is an uncontrolled and dysfunctional immune response involving the continuous activation and proliferation of lymphocytes and macrophages. Huang et al1 found that patients with COVID-19 who were admitted to the intensive care unit had higher plasma levels of cytokines, including interleukin (IL)–2, IL-7, IL-10, granulocyte-colony stimulating factor, IgG-induced protein 10 (also known as C-X-C motif chemokine 10), monocyte chemoattractant protein-1, macrophage inflammatory protein 1-alpha (also known as chemokine ligand 3), and tumor necrosis factor α. In the present study, we also found that markers of inflammatory response, such as C-reactive protein, procalcitonin, and leukocytes, were significantly increased among patients who suffered from cardiac injury. The activation or enhanced release of these inflammatory cytokines can lead to apoptosis or necrosis of myocardial cells.
In addition, preexisting cardiovascular diseases might also be more susceptible COVID-19–induced heart injury, as approximately 30% and 60% of patients with cardiac injury in the present study had a history of coronary heart disease and hypertension, respectively, which were significantly more prevalent than in those without cardiac injury. Similarly, in a recent report,2 25% and 58.3% of patients who were critically ill with COVID-19 had underlying heart diseases and hypertension, respectively. According to the “Diagnosis and Treatment of Novel Coronavirus Pneumonia (Trial Version 4),”17 elderly patients with underlying diseases are more likely to be infected with SARS-CoV-2 and tend to be severely ill, especially those with hypertension, coronary heart disease, and diabetes. Although there are few pieces of evidence to establish a direct association between cardiac injury and cardiovascular comorbidities, it is rational to presume that patients with coronary artery disease or heart failure are susceptible to cardiac injury, and once such patients are infected with severe pneumonia, myocardial ischemia or cardiac dysfunction are more likely to occur, ultimately leading to a sudden deterioration. On the other hand, acute inflammatory responses can also lead to ischemia in the presence of preexisting cardiovascular diseases. The inflammatory activity within coronary atherosclerotic plaques is exacerbated during systemic inflammatory response, making them prone to rupture.18 Inflammation also causes endothelial dysfunction and increases the procoagulant activity of the blood, which can contribute to the formation of an occlusive thrombus over a ruptured coronary plaque.19 Based on these lines of evidence, we hypothesize that an intense inflammatory response superimposed on preexisting cardiovascular disease may precipitate cardiac injury observed in patients with COVID-19 infections.
Some limitations existed in the present study. First, because of the logistical limitations at the onset of these emerging infections in Wuhan, some data, such as echocardiography data, electrocardiography data, and cytokine level measurements, were lacking from clinical examinations of patients in isolation wards or the intensive care unit, which limits the determination of potential mechanisms of cardiac injury. Second, because the clinical observation of patients is still ongoing, many with and without cardiac injury have not reached clinical end points. Third, data from larger populations and multiple centers are warranted to further confirm the outcomes of cardiac injury in COVID-19.
Cardiac injury is a common condition among patients hospitalized with COVID-19, and it is associated with a higher risk of in-hospital mortality. Although the exact mechanism of cardiac injury needs to be further explored, the findings presented here highlight the need to consider this complication in COVID-19 management.
Accepted for Publication: March 9, 2020.
Corresponding Author: Bo Yang, MD, PhD (firstname.lastname@example.org), and He Huang, MD, PhD (email@example.com), Cardiovascular Research Institute, Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Rd, Wuchang District, Wuhan 430060, Hubei, China.
Published Online: March 25, 2020. doi:10.1001/jamacardio.2020.0950
Author Contributions: Drs B. Yang and H. Huang had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Drs Shi, Qin, and Shen contributed to the work equally and should be regarded as co–first authors.
Concept and design: Shi, Qin, Shen, Cai, T. Liu, X. Liu, Liang, Zhao, H. Huang, B. Yang, C. Huang.
Acquisition, analysis, or interpretation of data: Shi, F. Yang, Gong, Liang, B. Yang.
Drafting of the manuscript: Shi, Qin, Shen, T. Liu, Zhao.
Critical revision of the manuscript for important intellectual content: Shi, Cai, F. Yang, Gong, X. Liu, Liang, H. Huang, B. Yang, C. Huang.
Statistical analysis: Shi, Qin, Shen, Cai, T. Liu, F. Yang, Gong.
Obtained funding: Shi.
Administrative, technical, or material support: Shi, Shen, F. Yang, Gong, Liang, Zhao, H. Huang, B. Yang, C. Huang.
Supervision: Shi, Qin, X. Liu, Liang, Zhao, B. Yang, C. Huang.
Conflict of Interest Disclosures: None reported.
Funding/Support: This research was supported by grants from the Nature Science Foundation of China (grants 81800447 and 81770324), the Nature Science Foundation of Hubei province (grant 2017CFB204), and the Major Program of Technological Innovation of Hubei Province (grant 2016ACA153).
Role of the Funder/Sponsor: The funders had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.
Additional Contributions: We acknowledge all medical staff involved in the diagnosis and treatment of patients with COVID-19 in Wuhan. We thank Xu Liu, MD, PhD, Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, for guidance in revision of manuscript and interpretation of results; we thank Min Chen, MAEng, Xuecheng Yu, MAEng, and and Zhongli Dai, BE, Wuhan Shinall Technology Co Ltd, for data collation and statistical analysis. They were not compensated for their contributions.
et al. Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China. JAMA
. Published online February 7, 2020. doi:10.1001/jama.2020.1585
et al; ARDS Definition Task Force. Acute respiratory distress syndrome: the Berlin Definition. JAMA
. 2012;307(23):2526-2533.PubMedGoogle Scholar
Kidney Disease: Improving Global Outcomes (KDIGO) Acute Kidney Injury Work Group. KDIGO clinical practice guideline for acute kidney injury. Kidney Int Suppl
. 2012;2:1.Google ScholarCrossref
et al. The single-cell RNA-seq data analysis on the receptor ACE2 expression reveals the potential risk of different human organs vulnerable to Wuhan 2019-nCoV infection. Front Med
. Published online February 8, 2020.Google Scholar
II. The hidden burden of influenza: a review of the extra-pulmonary complications of influenza infection. Influenza Other Respir Viruses
. 2017;11(5):372-393. doi:10.1111/irv.12470
et al. Fatal outcome of human influenza A (H5N1) is associated with high viral load and hypercytokinemia. Nat Med
. 2006;12(10):1203-1207. doi:10.1038/nm1477
S. Systemic infections cause exaggerated local inflammation in atherosclerotic coronary arteries: clues to the triggering effect of acute infections on acute coronary syndromes. Tex Heart Inst J
. 2007;34(1):11-18.PubMedGoogle Scholar