The data points are means, and the bars indicate 95% confidence intervals. Where appropriate, LDL-C measurements of patients who had returned to within 20% of their change from baseline LDL-C (and therefore no longer had available LDL-C values) were imputed with their individual baseline values.
Where appropriate, LDL-C measurements of participants who had returned to within 20% of their change from baseline LDL-C (and therefore no longer had available LDL-C values) were imputed with their individual baseline values.
Defined as days taken for individuals to return to within 20% of their change from baseline low-density lipoprotein cholesterol levels up to day 360.
Boxes represent the median and interquartile range of LDL-C values measured throughout the study, and whiskers represent the minimum and maximum measured values.
eTable 1. Number of patients whose LDL-C had not returned to 20%
eTable 2. Percentage of participants who did not return to within 20% of their change from baseline PCSK9 or LDL-C at Day 360
eTable 3. Median months of follow-up through which patients maintained a ≥ 39 mg/dL (1.0 mmol/L) and a ≥ 58 mg/dL (1.5 mmol/L) reduction in LDL-C
eTable 4. Adverse event rates to Day 360
eFigure 1. Design of the ORION-1 phase 2 trial of inclisiran
eFigure 2. Waterfall plots of individual percentage changes in LDL-C at Day 360 after a single or two-dose starting regimen for those who had not returned to within 20% of their change from baseline at Day 330 and thus returned for a final visit
eFigure 3. Mean and 95% confidence interval reduction in percentage LDL-C between Day 90 and Day 270
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Ray KK, Stoekenbroek RM, Kallend D, et al. Effect of 1 or 2 Doses of Inclisiran on Low-Density Lipoprotein Cholesterol Levels: One-Year Follow-up of the ORION-1 Randomized Clinical Trial. JAMA Cardiol. 2019;4(11):1067–1075. doi:10.1001/jamacardio.2019.3502
Can inclisiran reduce mean low-density lipoprotein cholesterol (LDL-C) exposure with an infrequent dosing regimen, thereby offering sustained LDL-C lowering with infrequent dosing?
In this prespecified analysis of a randomized clinical trial, 1 dose of inclisiran lowered time-averaged LDL-C levels over 1 year by 29.5% to 38.7%, and 2 doses at day 1 and day 90 lowered time-averaged LDL-C over 1 year by 29.9% to 46.4% in a dose-dependent manner. A 50% LDL-C reduction was maintained for at least 6 months after 2 doses of 300 mg on day 1 and day 90.
Through potent and durable lowering of LDL-C with a twice-a-year dosing regimen, inclisiran could offer a sustained lipid-lowering therapeutic option in the future.
Sustained reductions in low-density lipoprotein cholesterol (LDL-C) with lipid-lowering therapies that require frequent dosing are reliant on patient adherence, and poor adherence is associated with worse clinical outcomes.
To determine whether inclisiran, a small interfering RNA, reduces mean LDL-C exposure with an infrequent dosing regimen.
Design, Setting, and Participants
Prespecified analysis of a randomized, double-blind, placebo-controlled multicenter phase 2 clinical trial. Participants were followed up monthly for LDL-C levels and proprotein convertase subtilisin-kexin type 9 (PCSK9) measurements as well as safety until their LDL-C levels had returned to within 20% of their change from baseline (maximum 360 days). The study included patients with elevated LDL-C despite maximally tolerated statin therapy. Data were analyzed between January 11, 2016, and June 7, 2017.
One dose (200, 300, or 500 mg on day 1) or 2 doses (100, 200, or 300 mg on days 1 and 90) of inclisiran sodium or placebo.
Main Outcomes and Measures
Duration of time to return to within 20% of change from baseline for LDL-C levels and time-averaged LDL-C reductions over 1 year.
At baseline, among the 501 participants, 65% were men (n = 326 of 501), mean age was 63 years, 6% had familial hypercholesterolemia (n = 28 of 501), and 69% had established ASCVD (n = 347 of 501). Baseline LDL-C was 128 mg/dL among 501 randomized participants. The percentage of participants who were followed up to day 360 because their LDL-C levels had not returned to within 20% of their change from baseline ranged from 48.3% to 65.0% for those receiving a single dose and between 55.9% and 83.1% of those receiving 2 doses, with similar effects observed for PCSK9. Time-averaged reduction in LDL-C levels over 1 year after a single dose ranged from 29.5% to 38.7% (P < .001 between groups) and from 29.9% to 46.4% (P < .001 between groups) for those who received 2 doses. The 2-dose 300-mg regimen produced the highest proportion of responders at day 360 and the greatest mean reduction in LDL-C over 1 year. Incidence of adverse events was similar through to 1 year.
Conclusions and Relevance
Treatment with inclisiran resulted in durable reductions in LDL-C over 1 year. Inclisiran may offer a novel approach to LDL-C reduction with the convenience of infrequent dosing.
ClinicalTrials.gov identifier: NCT02597127
Convergence of data from cohorts, mendelian randomization studies, and randomized clinical trials demonstrate that low-density lipoprotein cholesterol (LDL-C) is not only a causal factor for atherosclerotic cardiovascular disease (ASCVD) but that the cumulative exposure to LDL-C during an individual’s lifetime is a major driver of ASCVD.1-4 Hence, lifelong LDL-C reductions from genetic variants confer a larger relative protection against ASCVD than similar reductions in LDL-C achieved with therapies initiated later in life with a shorter duration of exposure.1,5 Statins are the first-choice treatment across all lipid guidelines for the prevention of ASCVD, with the intensity of therapy being matched to the level of risk to achieve sustained reductions and thus lower cumulative exposure to LDL-C. Many patients who fail to achieve LDL-C goals require additional lipid-lowering therapies ranging from small molecules, such as ezetemibe, which require daily dosing or monoclonal antibodies that require a twice to once monthly dosing regimen. Add-on lipid-lowering therapy places an additional medication burden on the patient. In many areas of health care, higher medication burden has been associated with lower compliance with therapeutic regimens.6,7 Furthermore, at least for small molecules that require daily dosing, adherence is associated consistently with outcomes.8,9
Inclisiran is an investigational small interfering RNA that inhibits proprotein convertase subtilisin/kexin type 9 (PCSK9) synthesis in the liver and has a long duration of action, which may offer a convenient dosing regimen for patients who require significant LDL-C lowering. We have previously reported that a single dose of 300 mg of inclisiran sodium reduced LDL-C by 38.4% at 180 days of follow-up and a 2-dose starting regimen of inclisiran reduced it by 52.6%.10 Monthly follow-up in this phase 2 trial, ORION-1, continued for up to 1 year. The objective of this prespecified analysis was to describe the mean reduction in LDL-C exposure over 1 year achievable with a single-dose or 2-dose regimen of inclisiran, the within-individual variability in LDL-C, and the persistence of the therapeutic effect at 1 year, as well as overall safety.
The design of the ORION-1 trial protocol has been reported in detail previously.10 Briefly, ORION-1 was a randomized, double-blind, placebo-controlled phase 2 clinical trial to evaluate the efficacy, safety, and tolerability of different doses of inclisiran (eFigure 1 in the Supplement). Eligible participants had either a history of ASCVD with LDL-C higher than 70 mg/dL (to convert to millimoles per liter, multiply by 0.0259) or ASCVD risk equivalents (eg, type 2 diabetes or familial hypercholesterolemia) with LDL-C higher than 100 mg/dL despite maximally tolerated statin therapy. Patients were randomly assigned to a single dose of inclisiran sodium, 200, 300, or 500 mg, or placebo on day 1 or 2 doses of inclisiran sodium, 100, 200, or 300 mg, or placebo on day 1 and day 90.
The primary efficacy end point of ORION-1 was the percentage change in LDL-C from baseline to day 180. Participants whose LDL-C had not returned to within 20% of their change from baseline had additional monthly follow-up visits until this level had been reached up to a maximum of 360 days. A prespecified secondary end point was the duration of time to return to within 20% of their change from baseline for LDL-C and PCSK9. At each visit, LDL-C and PCSK9 levels, adverse events, and safety laboratory assessments were collected. The protocol and written informed consent documents were approved by the institutional review board or independent ethics committee for each clinical site.
All plasma samples were obtained in a fasting state and analyzed using the laboratory analytical methods described previously at Medpace Reference Laboratories, certified by the Lipid Standardization Part III Program of the US Centers for Disease Control and Prevention–National Heart, Lung, and Blood Institute (NHLBI).10 The LDL-C levels were measured using β quantification. The PCSK9 levels were measured using enzyme-linked immunosorbent assay.
Adverse events data, vital signs, clinical examinations, electrocardiograms, and laboratory values were obtained for all participants who received at least 1 dose of study drug until the last visit. Laboratory parameters included hematological parameters, renal function, liver function tests, and creatine kinase. Adverse events were classified as mild, moderate, or severe by the investigator with the use of standard Medical Dictionary for Regulatory Activities terms and according to system organ class.
The prespecified secondary study end point of duration of time to return to within 20% of change from baseline LDL-C was visually represented as waterfall plots for each individual in the trial. Additionally, we report the proportions of individuals who at day 360 had not returned to within 20% of their change from baseline LDL-C and PCSK9 after receiving inclisiran. The reduction in LDL-C at day 360 vs baseline was assessed quantitatively for statistical significance using paired t tests. In a post hoc analysis, we calculated the mean percentage change in LDL-C and PCSK9 over the entire first year for each of the dosing groups as the mean of the reductions observed monthly over 1 year. To estimate the mean percentage change in LDL-C potentially achievable with a twice-yearly dosing regimen, we additionally calculated the mean LDL-C reduction over the immediate 6 months following a second dose of inclisiran. Because absolute LDL-C reductions drive clinical benefit of lipid-lowering therapies, we calculated the median number of months that either a single or 2 doses of inclisiran provided LDL-C reductions of at least 39 mg/dL or at least 58 mg/dL up to a maximum of 1 year, which is when follow-up ended. Where appropriate, LDL-C measurements of participants who had returned to within 20% of their change from baseline LDL-C (and therefore no longer had available LDL-C values) were imputed with their individual baseline values. Hence, the primary analyses reported (Figures 1 and 2) provide conservative estimates, using imputed data where appropriate. For comparison, a per protocol analysis including only participants who completed all study-related measures at day 270 and at day 360 (responders) was performed as a sensitivity analysis and is reported separately only as text.
To assess interindividual variation in response at day 360, waterfall plots of individual percentage changes in LDL-C levels were created for those who had not returned to within 20% of the change from baseline at day 330 and thus returned for a final visit. To assess the effect of a single or 2 doses of inclisiran vs placebo on month-to-month variability in LDL-C levels as a reflection of usual LDL-C exposure for inclisiran vs placebo, we created box-and-whisker plots of on-treatment LDL-C levels (each box represents a patient’s median and interquartile range [IQR] of LDL-C throughout the study, and whiskers represent the patient’s maximum and minimum observed LDL-C values). However, because placebo-treated participants were only followed up until day 210, box-and-whisker plots reflect each participant’s repeated measure at days 30, 60, 90, 120, 150, 180, and 210. For convenience, we provide data on the 300-mg single or 2-dose regimens of inclisiran sodium and matching placebo.
We also performed a post hoc evaluation of adverse event frequencies during the extended follow-up period. Adverse event rates are presented for the overall safety population (all participants who received at least 1 dose of inclisiran or placebo).
Continuous variables were summarized using means and standard deviations or medians and IQR. Categorical variables at baseline were expressed as counts and percentages. Time courses are presented as means and 95% confidence intervals. Analyses were performed using SAS software, version 9.2 and higher (SAS Institute), with a P value of less than .05 considered significant using a 2-sided test.
The ORION-1 trial included 501 participants, 497 of whom received at least 1 dose of inclisiran or placebo. At baseline, among the 501 participants, 65% were men (n = 326 of 501), mean age was 63 years, 6% had familial hypercholesterolemia (n = 28 of 501), and 69% had established ASCVD (n = 347 of 501) (Table). At baseline, mean (SD) LDL-C was 128 (50) mg/dL, with 73% of participants receiving statin therapy (n = 365 of 501) and 31% participants receiving ezetimibe treatment (n = 151 of 501). All baseline characteristics were balanced between inclisiran or matching placebo within the single-dose and double-dose starting regimen groups. eTable 1 in the Supplement shows the number and proportion of participants at each follow-up visit after the day 210 end-of-study visit.
The time courses of percentage change in PCSK9 and LDL-C are shown through to day 360 (Figure 1). In the single-dose group, the percentage reduction in PCSK9 ranged from 17.5% to 29.7% at day 360 (Figure 1A), with corresponding reductions in LDL-C ranging from 15.4% to 21.6% at day 360 (Figure 1B; P < .001 for all groups at day 360). In the 2-dose group, the percentage reduction in PCSK9 ranged from 15.8% to 37.7% at day 360 (Figure 1B), and reductions in LDL-C ranged from 12.6% to 31.4% at day 360 (Figure 1B; P < .001 for all groups at day 360 from baseline). The greatest reductions in PCSK9 and LDL-C were observed with the 2-dose starting regimen of 300 mg.
The time-averaged reductions in PCSK9 levels from day 30 to day 360 ranged from 44.5% to 55.9% and from 43.1% to 60.5% for single-dose and 2-dose regimens (Figure 2). Reductions in LDL-C ranged between 29.5% to 38.7% and 29.9% and 46.4% for single-dose and 2-dose groups (Figure 2). The gain in time-averaged LDL-C reduction over 1 year with 500 mg vs 300 mg single dose was modest at 2.1% (36.6% vs 38.7%; P = .48). A second 300-mg dose administered at day 90 offered an additional reduction in LDL-C levels of approximately 10% (36.6% vs 46.4%; P = .002).
Time taken to return to within 20% of change from baseline LDL-C following a single dose or 2 doses of inclisiran is shown as waterfall plots in Figure 3. At day 180, among patients who received at least 1 dose of study drug and had both the baseline and the 180-day follow-up LDL-C assessment available, the percentage of patients who had a persistent response (ie, had not returned to within 20% of change from baseline LDL-C level) ranged between 90.0% (n = 54) and 100.0% (n = 60) and between 96.6% (n = 57) and 100.0% (n = 59) following a single dose or 2 doses of inclisiran. At day 330, the corresponding figures were 60.0% to 75.0% and 69.5% to 86.3% of those receiving a single dose or 2 doses, respectively. The duration of time to return to within 20% of change from baseline LDL-C was dose dependent and at day 360, most participants still had not returned to within 20% of their change from baseline LDL-C and PCSK9 levels (eTable 2 in the Supplement). eFigure 2 in the Supplement shows waterfall plots of individual percentage change in LDL-C levels at day 360.
Patients maintained an LDL-C reduction of at least 39 mg/dL from baseline for a median of 6 to 9 months (P < .001 for 500 mg vs 200 mg) with a single dose and from 5 to 10 months with 2 doses of inclisiran (P < .001 for 100 mg vs 300 mg) (eTable 3 in the Supplement). In the study cohort, an LDL-C reduction of at least 58 mg/dL from baseline was maintained from 2 to 5 months (P = .001 for 500 mg vs 200 mg) and between 1 to 6 months (P < .001 for 300 mg vs 100 mg for those receiving a single dose or 2 doses of inclisiran) (eTable 3 in the Supplement).
Month-to-month variation in LDL-C levels between day 30 and day 210 for every patient who received either placebo or a 300-mg dose of inclisiran sodium as a single dose or 2 doses on day 1 and day 90 are shown in Figure 4 as the median, IQR, and minimum-measured and maximum-measured LDL-C levels during the treatment window. One dose of 300 mg of inclisiran sodium administered on day 1 or 2 doses on days 1 and 90 appeared in general to not only reduce LDL-C levels more than placebo but to maintain lower LDL-C levels consistently despite the infrequent dosing regimen.
In the sensitivity analyses (ie, including only patients with available measurements), reductions in LDL-C at day 270 and day 360 in the single-dose groups ranged between 25.3% and 32.4% and between 29.8% and 28.4%, respectively. In the 2-dose groups, the corresponding reductions ranged between 25.2% and 43.4% and between 17.6% and 33.8%, respectively. The time-averaged LDL-C reductions over 1 year ranged between 31.6% and 39.8% in the single-dose groups. For the 2-dose groups, the time-averaged LDL-C reductions over 1 year ranged between 31.0% and 46.8%.
The greatest reduction in LDL-C appeared to occur with the 300-mg 2-dose regimen. Following the second 300-mg injection at day 90, the percentage reduction in LDL-C at day 270 was similar to the reduction at day 90 after a single dose. The reduction in LDL-C between day 90 and day 270 after a single or 2 300-mg doses are shown in eFigure 3 in the Supplement. For the single dose, the mean reduction in LDL-C declined predictably but steadily, with a time-averaged LDL-C reduction between days 90 and 270 of 39%. Among those, given the second dose at day 90, the time-averaged reduction over the same period was 50% (P value for comparison <.001).
Adverse event rates are shown in eTable 4 in the Supplement. Adverse event rates in placebo-treated and inclisiran-treated patients were generally similar to the end-of-study visit at day 210 and stable in inclisiran-treated patients during continued follow-up to day 360. There were 2 deaths within the first 210 days that were not attributed to inclisiran by the investigators. There were no further deaths in the 360-day observation window.
This analysis of the ORION-1 trial extends our prior observations through to 1 year and provides the largest and longest safety data set, to our knowledge, to date for a small interfering RNA in any given therapeutic area. We observed that 1 year after administration of a single dose of inclisiran there was a dose-dependent persistence of response with approximately 50% to 65% of participants not having returned to within 20% of their change from baseline LDL-C. Following a second dose of inclisiran at day 90 the proportion of participants whose response persisted at day 360 increased also in a dose-dependent fashion from 48% (n = 29 of 60) and was highest overall (83%; n = 49 of 59) with the 300-mg 2-dose starting regimen. Notably, the lowest of the 2-dose starting regimen (100 mg) was comparable with the persistence of response observed in the 2 highest single-dose regimens (300 mg or 500 mg).
The mean reduction in PCSK9 levels over 1 year ranged from 44.5% to 55.9% with a single dose and from 43.1% to 60.5% with 2 doses of inclisiran. This translated into a time-averaged reduction in LDL-C levels of 30% and 39% for a single 200-mg and 500-mg injection over 1 year and a 30% and 46% reduction for 100 mg and 300 mg administered at day 1 and day 90. The rate at which LDL-C returned toward baseline was similar after repeated dosing. The 500-mg dose did not materially increase the magnitude or the duration of response compared with 300 mg. In contrast, lower dosing regimens of inclisiran resulted in more modest reductions in time-averaged LDL-C and PCSK9. Hence, the 300-mg dose appears to have the optimal profile. Following the second 300-mg dose at day 90, the median reduction in LDL-C over the following 180 days was 50%. Therefore, it is estimated that following the 2-dose starting regimen of 300 mg administered on days 1 and 90, it should be feasible for maintenance doses of 300 mg to be administered 180 days apart from day 270, which would allow for a twice-a-year dosing regimen after the second dose. This regimen is being evaluated in an ongoing pivotal registration program, which has enrolled approximately 3600 participants (NCT03397121, NCT03399370, and NCT03400800).
We previously reported safety data to day 210. During the additional 150 days of safety follow-up observation through to 1 year, there were no additional deaths observed. The adverse event profile and the incidence of adverse events were similar across placebo-treated and inclisiran-treated participants without notable safety observations through to day 360. With longer exposure, the proportions of events observed at day 360 were not dissimilar to those reported to day 210.
A potential advantage of inclisiran compared with therapies with a shorter duration of action is the relative stability in achieved LDL-C levels over time that can be achieved despite the infrequent dosing regimen. When given in addition to statin therapy, inclisiran could theoretically attenuate short-term fluctuations in LDL-C that may result from intermittent statin use (eg, owing to poor adherence) because those fluctuations will occur from a lower baseline. Variability in LDL-C over time, even among patients prescribed stable doses of high-intensity statins, has been reported and may be associated with poor adherence.10 In our previous analysis of the difference in LDL-C at day 180 as compared with baseline among patients randomized to placebo injections, LDL-C values among individuals who were receiving a stable background dose of lipid-modifying therapy (per protocol prior to study entry) varied considerably. For instance, between these 2 times, among the placebo group, some patients’ LDL-C levels went up by as much as 40% while others’ went down by as much as 40%.10 About half the placebo group had an LDL-C rise while the other half had a fall, and the mean change (difference) in LDL-C levels over these 2 times in the overall placebo group was approximately zero. Such variability is unlikely to result from natural biological variation among individuals receiving stable dosing regimens and likely to result from variability in adherence between 2 times. Importantly, during a period of 5 or more years of treatment, those with the greatest variability in LDL-C levels have associated worse cardiovascular outcomes,11,12 with each 1 SD increase in visit-to-visit LDL-C variability associated with a 16% increased risk of coronary events independent of achieved LDL-C levels.11 Large observational studies have also shown that greater variations in lipids are associated with higher risk of ASCVD and all-cause mortality.13 Genetic studies and trial data have shown that when scaled for magnitude and duration, the benefit of lowering LDL-C through different pathways is similar. This suggests that benefits depend on LDL-C exposure (reduction) rather than the mechanism by which it is achieved.1,14 It could be speculated that any therapy that conveniently attenuates absolute month-to-month fluctuations in LDL-C over a long time offers advantages vs regimens that require much more rigorous adherence to treatment regimens to achieve the same. The ongoing ORION-4 cardiovascular outcomes trial (NCT03705234) could provide a hint as to whether the more stable LDL-C reductions produced by inclisiran may yield greater benefit than would be expected for any given reduction in LDL-C at a fixed point in time. Because poor adherence contributes to real-world fluctuations in LDL-C levels and good adherence is often artificially inflated in the context of clinical trials, it will be impossible to draw conclusions about the benefits of an infrequent dosing regimen until real-world evidence becomes available.
Nonstatin lipid-lowering therapies, such as ezetimibe and monoclonal antibodies to PCSK9, improve outcomes when added to statins proportional to the absolute reduction in LDL-C levels and the duration of therapy, with around one-fifth lowering of risk per 39 mg/dL reduction in LDL-C levels.4,14-16 In contrast to these large outcome studies, our study was not powered for outcomes, but we did demonstrate the potential for inclisiran to sustain clinically meaningful reductions in absolute LDL-C levels with an infrequent dosing regimen. A large cardiovascular outcomes trial (NCT03705234) is testing whether lowering LDL-C with inclisiran reduces cardiovascular outcomes.
There are several limitations that merit consideration. First, because the placebo group was not followed up beyond day 210, no direct comparisons to placebo can be made at day 360. Second, patients whose LDL-C levels had returned to within 20% of their change from baseline were no longer followed up after day 210, which limits the number of patients with available data. A conservative approach was taken to estimate the effect of inclisiran on LDL-C by assuming that all patients without available measurements had returned to baseline. This assumption did not materially affect the results as demonstrated by the sensitivity analysis, which included only patients with available data (ie, best-case analysis). Notably, all patients in the 300-mg 2-dose group with measurement data at day 240 continued to be followed up through to day 360. Although this analysis extends the observation period to 1 year of follow-up, results from the larger and longer ongoing ORION-9, ORION-10, and ORION-11 trials are required to confirm the findings of this analysis. Finally, further data on longer-term safety, adherence/persistence, and clinical event reduction are required.
In conclusion, a single 300-mg dose of inclisiran sodium provides a mean reduction in LDL-C of 36.6% over 1 year, and a 50% LDL-C reduction in LDL-C can be maintained for at least 6 months after 2 doses of 300 mg at day 1 and day 90. Therefore, inclisiran administered at day 1, day 90, and twice a year thereafter should enable patients to achieve stable and meaningful reductions in LDL-C. The ongoing phase 3 trials of inclisiran will provide longer-term follow-up data in larger numbers of patients.
Corresponding Author: Kausik K. Ray, FRCP, Imperial Centre for Cardiovascular Disease Prevention, Department of Primary Care and Public Health, Imperial College London, Charing Cross Hospital, Reynolds Building, Room 320, London W68RF, England (firstname.lastname@example.org).
Accepted for Publication: July 26, 2019.
Published Online: September 25, 2019. doi:10.1001/jamacardio.2019.3502
Author Contributions: Dr Ray had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.
Concept and design: Ray, Stoekenbroek, Kallend, Leiter, Landmesser, Wijngaard, Kastelein.
Acquisition, analysis, or interpretation of data: All authors.
Drafting of the manuscript: Ray, Stoekenbroek, Kallend, Nishikido, Wright.
Critical revision of the manuscript for important intellectual content: Ray, Stoekenbroek, Kallend, Leiter, Landmesser, Wright, Wijngaard, Kastelein.
Statistical analysis: Stoekenbroek, Kallend, Wijngaard.
Administrative, technical, or material support: Ray, Stoekenbroek, Kallend, Nishikido.
Supervision: Ray, Stoekenbroek, Kallend, Landmesser, Wright, Wijngaard, Kastelein.
Conflict of Interest Disclosures: Dr Ray reported personal fees from The Medicines Company and support from the National Institute of Health Research Imperial Biomedical Research Centre during the conduct of the study; grants from Amgen, Sanofi, Regeneron, Pfizer, MSD; and personal fees from Amgen, Sanofi, Regeneron, MSD, Esperion, Resverlogix, Kowa, BI, Novo Nordisk, AKCEA, Cerenis, AbbVie, Eli Lilly, Silence Therapeutics, Bayer, Daiichi Sankyo, Algorithm, Cipla, Dr Reddys, AstraZeneca, Pfizer, and Zueling Pharma outside the submitted work. Dr Stoekenbroek reported being an employee and shareholder of The Medicines Company. Dr Kallend reported personal fees and other support from The Medicines Company during the conduct of the study and outside the submitted work; in addition, Dr Kallend has a patent to Methods for Preventing Cardiovascular Events through PCSK9 protein reduction pending. Dr Nishikido reported grants from the Uehara Memorial Foundation outside the submitted work. Dr Leiter reported grants and personal fees from The Medicines Company during the conduct of the study; personal fees from AstraZeneca, Esperion, Merck, and Sanofi/Regeneron; and grants and personal fees from Amgen and Kowa outside the submitted work. Dr Landmesser reported personal fees from The Medicines Company during the conduct of the study and personal fees from Amgen and Sanofi outside the submitted work. Dr Wright reported personal fees and other support from The Medicines Company, Sanofi-Regeneron, and AstraZeneca outside the submitted work. Dr Wijngaard reported other support from The Medicines Company during the conduct of the study and outside the submitted work. Dr Kastelein reported personal fees from The Medicines Company during the conduct of the study and personal fees from Amgen, Sanofi Aventis, Regeneron, CiVi Biopharma, and Draupnir outside the submitted work. No other disclosures were reported.
Funding/Support: This study was funded by The Medicines Company.
Role of the Funder/Sponsor: The trial was designed by the principal investigator (Dr Ray) and the sponsor, the Medicines Company, and was performed by the sponsor and World Wide Clinical Trials. Data were collected by the investigators, and data analyses were conducted by Statistics Collaborative and The Medicines Company. Three coauthors are employees of the funders and contributed to the analysis and interpretation of the data. The funding source had no role in the preparation, review, or approval of the manuscript and decision to submit the manuscript for publication.
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