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Dec 2011

Sensitivity of Immunofluorescence Studies vs Enzyme-Linked Immunosorbent Assay for Diagnosis of Bullous Pemphigoid

Author Affiliations

Author Affiliations: Molecular and Cell Biology Laboratory, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico (IDI-IRCCS), Rome, Italy (Drs Di Zenzo and Zambruno); Department of Dermatology, Rouen University Hospital, University of Rouen, Rouen, France (Dr Joly); and Department of Dermatology, University Hospital –Inselspital, Berne, Switzerland (Dr Borradori).

Arch Dermatol. 2011;147(12):1454-1456. doi:10.1001/archderm.147.12.1454-b

We read with interest the Practice Gap comments of Dr Chan published in the March issue of the Archives.1 We agree that there is a lack of consensus on diagnostic criteria for bullous pemphigoid (BP) and a need to provide better information in this area. However, the author's recommendation to routinely perform the commercially available enzyme-linked immunosorbent assay (ELISA) for BP180 instead of using indirect immunofluorescence (IIF) microscopy studies on a split-skin sample for the diagnosis of BP cannot be fully supported.