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August 1998

Immunocompetent Cells and Adhesion Molecules in 14 Cases of Cutaneous Drug Reactions Induced With the Use of Antibiotics

Arch Dermatol. 1998;134(8):1040-1041. doi:

Having previously published the immunohistological characteristics of amoxicillin-induced rashes,1 we report herein a similar study performed in patients with cutaneous adverse drug reactions (ADRs) related to the use of antibiotics.

Prior to the administration of any immunomodulating therapy, skin biopsy samples were obtained from 14 patients (6 men and 8 women; mean age, 64 years) 1 to 12 days after the onset of antibiotic-induced cutaneous ADR (Table 1): maculopapular rash (MPR) induced with the use of β-lactams (5 patients), norfloxacin (1 patient), isoniazid (1 patient), or pristinamycin (3 patients), and 4 cases of erythroderma2 induced with the use of pristinamycin. For each patient indirect immunofluorescence was performed as previously described.1 Langerhans cells were visualized using monoclonal antibodies to CD1a and HLA-DR (Coulterclone, Hialeah, Fla). T-cell subsets and their activation were determined using antibodies against CD3, CD4, CD8, HLA-DR, and CD25 (Coulterclone). The expression of a series of adhesion molecules was investigated using monoclonal antibodies against CD11a to CD18 (leukocyte function–associated antigen 1), CD54 (intercellular adhesion molecule 1), CD106 (vascular cell adhesion molecule 1 [Immunotech, Luminy, France]), CD62E (endothelial leukocyte adhesion molecule 1 [E-selectin]), CD62L (leukocyte endothelial cell adhesion molecule 1 [L-selectin]), and CD62P (P-selectin [GMP 140, Becton Dickinson, Mountain View, Calif]).

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