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February 2005

Molecular Diagnosis of Cutaneous Diseases

Author Affiliations

Author Affiliations: Center for Clinical Studies (Dr Sra) and Department of Dermatology, University of Texas Health Science Center (Drs Rady, Shipley, and Tyring), Houston; Texas Tech School of Medicine, Lubbock (Ms Babb-Tarbox and Mr Aboutalebi); Department of Dermatology, Houston Advanced Research Center, The Woodlands, Tex (Dr Dao).

Arch Dermatol. 2005;141(2):225-241. doi:10.1001/archderm.141.2.225

Objectives  To provide an update on the molecular procedures used increasingly in the study and diagnosis of a variety of dermatologic malignancies and inflammatory disorders and to explore the potential use of these techniques in clinical dermatology. Herein, we review assays such as G-banding, fluorescence in situ hybridization, comparative genomic hybridization, and spectral karyotyping in conjunction with the polymerase chain reaction and DNA microarrays.

Data Sources  PubMed was searched for published articles on molecular diagnosis and dermatologic diseases.

Study Selection  All English-language studies were selected if they provided useful methodologic information or highlighted the usefulness of molecular techniques.

Data Extraction  Only methodologic and qualitative information was extracted.

Data Synthesis  The information was synthesized into 2 sections: one describing the principles of different molecular diagnostic techniques, and the other highlighting the contributions of molecular diagnostic techniques to the understanding and diagnosis of several dermatologic diseases.

Conclusions  A basic understanding of the principles of molecular diagnostic techniques is crucial for the practicing dermatologist to benefit from the increasing number of molecular diagnostic articles appearing in the literature and potentially to apply these methods in clinical practice.

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