Human cutaneous leishmaniasis (HCL) has been known in Spain since 1914, when it was described in the Alpujarras region (Andalusia). The causative agent was characterized in 1986 for the first time by isoenzyme electrophoresis and identified as a zymodeme of Leishmania infantum.1 Further studies showed the enzymatic polymorphism of L infantum in HCL, which we now report in Catalonia.