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Article
October 1921

STAINING OF SPIROCHAETA PALLIDA BY THE FONTANA-TRIBONDEAU METHOD: ELIMINATION OF HEAT

Author Affiliations

Of the Calixto García Hospital and the Covadonga Sanatorium Laboratories HAVANA, CUBA

Arch Derm Syphilol. 1921;4(4):448-450. doi:10.1001/archderm.1921.02350230037002
Abstract

Our chief purpose in beginning our investigation was to discover a quick and reliable method, which could be employed by any one familiar with microscopic work, to demonstrate Spirochaeta pallida in the transudate of a chancre as easily as the tubercle bacillus is demonstrated in the sputum.

Nowadays, as we gain a better knowledge of the invasion of the tissues by the spirochete, when a patient with a so-called second syphilitic incubation is considered to have a possible case of the dreaded nervous involvement, it is convenient for the specialist to have a simple, reliable method to diagnose and to differentiate the chancre. With this in mind we searched for a staining method which should fill these requirements.

Our advocacy of this method does not mean that we consider it superior to the dark-field method, since both methods have their own advantages, but not all laboratories are equipped for dark-field

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