Further studies on the precipitation reaction for syphilis proposed by the author1 suggested several changes in the procedure which appeared to enhance considerably the value of the test. It will be recalled that in the original method a cholesterinized antigen was employed, and because of its high sensitiveness the test was completed after four hours' incubation. In the new procedure a noncholesterinized antigen is also employed. The same alcoholic extract of heart muscle is used with and without the addition of cholesterin. When employing the noncholesterinized antigen, however, it was observed that the rapidity of precipitation is not as marked as in the case of the cholesterinized one. It was necessary to incubate the tests with the former antigen over night in order to elicit complete precipitation in most cases. On the basis of this finding, and because of another consideration to be discussed presently, it was deemed best
KAHN RL. A SIMPLE QUANTITATIVE PRECIPITATION REACTION FOR SYPHILIS: THIRD COMMUNICATION. Arch Derm Syphilol. 1922;6(3):332–341. doi:10.1001/archderm.1922.02360030069005
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