One of the disadvantages inherent in the Wassermann test depends on the use of the hemolytic system as a measure of the reaction between the lipoid antigen and the lipoidophilic reagin in the patient's blood. The resulting hemolysis may be influenced by other factors than the one which it is intended to measure. Thus the patient's serum may contain substances hemolytic for sheep's blood, native hemolysins, or it may contain substances which are alone destructive to complement, anti-complementary substances. Various controls for these variable features of serum are employed, but are not wholly satisfactory. Another most serious difficulty with the hemolytic system is due to the somewhat uncertain quality of the fresh guinea-pig serum, the activity of which varies for different animals and for the same animal at different times. After removal from the animal, the cytase or complement is subject to natural deterioration, and the extent to which this
BAYLIS AB, SHEPLAR AE, MacNEAL WJ. SIMULTANEOUS VERNES AND WASSERMANN TESTS FOR SYPHILIS. Arch Derm Syphilol. 1924;10(3):306–313. doi:10.1001/archderm.1924.02360270035007
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