The laboratory tests on which this paper is based were the Wassermann, the Kahn and the glycerol-cholesterol precipitation.
The technic for the Wassermann test is that published by Hinton.1 Two antigens were used separately: one, a simple, cholesterolized, alcoholic extract of the heart of a guinea-pig and the other, an alcoholic extract of the heart of a human being half saturated with cholesterol at 17 C. The hemolytic system consisted of 2 units of amboceptor, 2 units of complement and 0.5 cc of a 5 per cent suspension of sheep's corpuscles. One-tenth cubic centimeter of serum was used for each antigen, and 0.2 cc. for the anticomplimentary control. The primary incubation was for forty minutes at 37 C. and the secondary incubation for one hour at the same temperature. Tests were read immediately after the secondary incubation. The results were reported as positive, negative and doubtful.
The Kahn reaction
SMITH CM. THE HINTON GLYCEROL-CHOLESTEROL PRECIPITATION REACTION IN SYPHILIS. Arch Derm Syphilol. 1929;19(3):439–448. doi:10.1001/archderm.1929.02380210094007
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