Although thirty years have elapsed since Plato and Neisser first prepared trichophytin and studied its diagnostic application in fungous diseases of the skin, the question of the specificity and the diagnostic value of the trichophytin reaction is still under discussion. Several trichophytins have been made by various authors from different fungi, but a standardized and universally recognized preparation is still lacking. Under such circumstances we felt justified in making a preparation of our own of recently isolated strains of fungi of common occurrence in our clinic. In our extract Trichophyton violaceum, Microsporon audouini and Epidermophyton interdigitale were used. These strains were grown separately in liquid medium— Sabouraud's broth (dextrose massée, 4 per cent; peptone, 1 per cent in water). After solution of all the materials, the medium was filtered through paper, distributed in several 250 cc. flasks, each of which contained about 150 cc. of medium, and sterilized
MUSKATBLIT E, DIRECTOR W. THE TRICHOPHYTIN TEST: REPORT OF THREE HUNDRED AND FIFTY CASES. Arch Derm Syphilol. 1933;27(5):739–744. doi:10.1001/archderm.1933.01450040748002
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