One of us1 recently described a general method for the preparation of bacterial antigens which consists essentially in the avoidance of all heat or chemical treatment. The cells are grown in mass cultures, are then washed free from metabolites in isotonic solution and are ground in a special type of ball mill2 which effectively breaks the bacteria into fragments. The emulsion prepared in this way is then filtered through a special ultrafilter,3 and the filtrate is employed prophylactically and therapeutically. The procedure outlined obviates, to a considerable degree, the reactions of denaturation which occur when the bacterial proteins are exposed to heat or to chemical treatment. And since, as would be expected, denaturation of the antigen is reflected in an altered response of the antibodies of the host, such undenatured antigens have been employed with considerably more clinical success than has attended the use of orthodox vaccines.
KRUEGER AP, TEMPLETON HJ. PREPARATION OF TRICHOPHYTIN. Arch Derm Syphilol. 1934;30(1):9–10. doi:10.1001/archderm.1934.01460130017003
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