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Article
February 1935

THE "MEMBRANE METHOD" FOR DETERMINING THE FUNGICIDAL ACTION OF CHEMICALS: ITS CLINICAL IMPLICATIONS

Author Affiliations

NEW YORK

From the chemical laboratory of the Beth Israel Hospital, and the Departments of Dermatology of the Beth Israel Hospital, and the New York University, University and Bellevue Hospital Medical College.

Arch Derm Syphilol. 1935;31(2):217-223. doi:10.1001/archderm.1935.01460200061007
Abstract

In 1931, in collaboration with Dr. E. Muskatblit, I offered a preliminary report on a newly conceived method for the determination of the fungicidal property of chemicals.

In place of the usual procedure for effecting fungistasis by the direct contamination of growth mediums with the chemicals to be tested, or of that for effecting fungicidity by the momentary immersion of fungus masses in solutions of these chemicals, we incorporated the chemicals in collodion and lined culture tubes with a thin film of the collodion which had been thus impregnated. After this film of collodion had thoroughly dried, the culture medium was poured into the tube and allowed to solidify in a slant. The surface of the medium was then seeded with a fungus mass and the culture allowed to grow at room temperatures. Under these conditions the chemicals to be tested were, at the outset at least, brought into touch

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