In 1931, in collaboration with Dr. E. Muskatblit, I offered a preliminary report on a newly conceived method for the determination of the fungicidal property of chemicals.
In place of the usual procedure for effecting fungistasis by the direct contamination of growth mediums with the chemicals to be tested, or of that for effecting fungicidity by the momentary immersion of fungus masses in solutions of these chemicals, we incorporated the chemicals in collodion and lined culture tubes with a thin film of the collodion which had been thus impregnated. After this film of collodion had thoroughly dried, the culture medium was poured into the tube and allowed to solidify in a slant. The surface of the medium was then seeded with a fungus mass and the culture allowed to grow at room temperatures. Under these conditions the chemicals to be tested were, at the outset at least, brought into touch
SHARLIT H. THE "MEMBRANE METHOD" FOR DETERMINING THE FUNGICIDAL ACTION OF CHEMICALS: ITS CLINICAL IMPLICATIONS. Arch Derm Syphilol. 1935;31(2):217–223. doi:10.1001/archderm.1935.01460200061007
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