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In the preparation of antigens from certain pathogenic fungi it is expedient to grow the organisms in a medium with a specified chemical composition in order to insure uniformity between mediums prepared at different times. Chemical mediums, such as buffered solutions of sucrose and nitrate, are unsatisfactory for producing continuous and abundant growth in culture of many species of pathogenic fungi. Mediums containing amino-acids, such as asparagine, produce abundant growth, but because of their cost they are not practical from an economic point of view. A medium composed of urea and dextrose has fulfilled the requirements of profuse growth and of slight cost.
The preparation of this medium, which may prove of interest to mycologists, is as follows:
Dissolve the constituents, except the urea, in 1,000 cc. of water. Put 250 cc. of solution into each of four small mouthed bottles. Sterilize the bottles in the autoclave at 15 pounds'
SOUTHWORTH WH. A SPECIFIC CHEMICAL MEDIUM FOR PATHOGENIC FUNGI. Arch Derm Syphilol. 1937;36(2):302. doi:10.1001/archderm.1937.01480020058007
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