Many dermatologists and laboratory workers will welcome a simple and satisfactory method of staining cutaneous scales and hair for an accurate determination of the presence or absence of fungi. Although a number of methods have been suggested and used by different mycologists, none of these methods has proved to be entirely satisfactory. Sabouraud1 defatted scales and hair in chloroform, boiled them two to three minutes in formic acid, washed them in distilled water, stained them one minute in Sahli's blue, washed and dehydrated them and mounted them in balsam. Beauverie and Hollande2 fixed hair and scales in a mixture of alcohol, ether and acetic acid, hydrated them, stained them for ten minutes in polychrome methylene blue, differentiated them in a mixture of glycol and creosote in water, dehydrated them and mounted them in balsam. The chief disadvantage of both of these methods lies in the fact that during
BERBERIAN DA. A METHOD OF STAINING HAIR AND EPITHELIAL SCALES. Arch Derm Syphilol. 1937;36(6):1171–1175. doi:https://doi.org/10.1001/archderm.1937.01480060044006
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