The importance of study of subsurface growth has not been realized. Frequently the only site from which fruiting bodies can be recovered is the base of a surface mycelial colony. This suggests the possibility that mediums producing subsurface growth would yield more amply fruiting bodies.
Staining pathogenic fungi during growth has not been given adequate attention. The incorporation of acid dyes or of low concentrations of basic dyes1 in mediums results in beautifully contrasted colonies and improves the clarity of the microscopic picture. Miyake2 found that 0.2, 0.5 and 1 per cent Eosin bläulich (G) could be incorporated in Sabouraud's proof medium and would serve well as a vital stain for the five pathogenic fungi he examined, without affecting their growth.
Previous studies3 have shown that subsurface growths can be obtained by using amino acids or hydrolyzed hair or skin as the source of nitrogen, and dextrose
WILLIAMS JW. SUBSURFACE MYCELIUM AND DYES: THEIR SIGNIFICANCE IN MYCOLOGIC STUDIES. Arch Derm Syphilol. 1938;38(2):235–240. doi:10.1001/archderm.1938.01480140075009
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