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May 1944

ACID ORCEIN-GIEMSA STAIN (Modification of Unna-Taenzer Method): A Useful Routine Stain for Dermatologic Sections

Author Affiliations

Monroe, Mich.

From the Department of Dermatology of the Monroe Hospital.

Arch Derm Syphilol. 1944;49(5):355-356. doi:10.1001/archderm.1944.01510110053014

Staining of sections of pathologic tissue with hematoxylin and eosin is probably the most widely used method throughout the United States and possibly the world. It has the advantages of simplicity, great reliability and clear color contrast, and it is satisfactory for most routine purposes. It is not sufficient, however, for dermatologic diagnosis, and different laboratories use a variety of other stains more or less routinely in addition to hematoxylin and eosin.

The procedure which in my experience gives the most information about the greatest variety of factors important for dermatopathologic diagnosis is the Unna-Taenzer method, combining the demonstration of elastic fibers by acid orcein solution with staining of cells by Unna's polychrome methylene blue solution.1 This method, however, is rather difficult to use and requires considerable experience and close supervision. I have found substitution of Giemsa's solution for the polychrome methylene blue solution

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