In a previous communication1 it was demonstrated that proteins, such as cottonseed, when applied topically are absorbed through the skin of human beings and of monkeys. In that study a site on the skin of the subject to be tested was passively sensitized to cottonseed antigen. After twenty-four to forty-eight hours, the antigen, suspended in a grease base, was rubbed in at another cutaneous site. The development of a wheal at the sensitized site marked the entrance of detectable amounts of antigen into the circulation.
The serum for sensitization was obtained from a patient strongly sensitive to cottonseed and with an extremely high titer of reagins for this antigen (1: 512 by the dilution method of Coca and Grove2). The serum was used in a dilution of 1:4 in human subjects and undiluted in monkeys. From 0.05 to 0.1 cc. was injected intracutaneously for local passive
WALZER A, SACK SS. CUTANEOUS ABSORPTION: II. THE VALUE OF PETROLATUM, ANHYDROUS WOOL FAT AND OTHER BASES IN PERCUTANEOUS ABSORPTION OF TOPICALLY APPLIED COTTONSEED ALLERGEN. Arch Derm Syphilol. 1944;49(6):427–431. doi:10.1001/archderm.1944.01510120041010
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