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Since most dermatologists have had or will have only an occasional opportunity to observe some tropical cutaneous diseases, it is important to secure as adequate confirmatory laboratory diagnosis as possible. In tropical medicine the laboratory plays an important role. Often standard texts do not include the practical details for the laboratory diagnosis.
Some general considerations may be given at first. Since parasites may often be few, perseverance and persistency are needed in the search for them. The casual glance or the examination of only a small portion of the slide preparation is entirely inadequate for the conclusion that the result of a test is negative. A systematic search of the slide should be made; multiple examinations should be done, and examinations should be repeated at varying intervals if indicated. When slides are used, it is important that they be clean; otherwise the preparation may not
GOLDMAN L. PREPARATION OF MATERIAL FOR LABORATORY DIAGNOSIS OF SOME TROPICAL DISEASES OF THE SKIN. Arch Derm Syphilol. 1944;50(4):264–266. doi:10.1001/archderm.1944.01510160036014
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