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Article
December 1944

COMBINED BODIAN AND MASSON STAINING METHOD APPLIED TO SKIN

Author Affiliations

LOS ANGELES

Arch Derm Syphilol. 1944;50(6):361-NP. doi:10.1001/archderm.1944.01510180009002
Abstract

The preparation of sections of skin with Bodian's1 silver protein (protargol) method, counterstained with Goldner's2 tetrachrome modification of Masson's3 trichrome method or its equivalent, yields interesting results, making the combined method4 of practical value on special occasions. It differs from the routine tetrachrome procedures in substituting protargol for hematoxylin. Protargol makes possible the simultaneous demonstration of melanin (black),5 epithelium and nuclei in general much more effectively than is the case with reduced silver or silver nitrate, which are inferior to protargol as general tissue methods. Nerve elements are visualized as effectively as when the method is applied to primarily neurologic tissue; in the method presented, however, fixation and staining technic are kept simple in order to be practical; the specific demonstration of nerve endings in skin may require more detailed attention, particularly regarding fixation. Such special fixation not only entails extra labor but

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