The method usually employed to preserve a specimen of a fungus colony which has been prepared for microscopic study is to "ring" the edge of the cover slip with balsam, petrolatum, de Khotinsky cement, melted shellac or some similar preparation. With this method it is difficult to prevent the formation of small cracks through which the fluid of the specimen evaporates in a few weeks or months. Also, the cover slip is not firmly attached to the slide, and gentle handling is necessary to avoid dislodging and destroying it. Such specimens usually become worthless after a short time, and students are handicapped unless fresh mounts are prepared each time they are desired.
By placing the specimen between a cover slip of the conventional size (22 mm.) and a smaller one (15 mm. or less, round or square) and mounting both as a unit in balsam, one can make
Wilson JW. AN IMPROVED METHOD FOR PREPARING PERMANENT SLIDES OF FUNGUS CULTURES. Arch Derm Syphilol. 1945;52(4):267. doi:10.1001/archderm.1945.01510280051012
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