CULTURES of the leishmania parasite were first made by Nicolle,1 and the customary method is to use on the N. N. N. (Nicolle Novy-McNeal) medium. Culture is, however, as a rule, used for laboratory transmission and therapeutic experiments while only rarely has it been employed as an aid in clinical diagnosis.
In countries where the disease occurs endemically there are always numerous cases which, although clinically they may arouse the suspicion of leishmaniasis, require confirmation by objective methods by which the parasite can be demonstrated. This applies particularly to the ulcerative lesions of long standing and to relapsing leishmaniasis, in which it is generally impossible to demonstrate the parasites in smear preparations. In these recurrences which clinically show a striking resemblance to lupus and to tertiary syphilis and in which it is equally difficult to discover the parasites, it is of greatest importance to be in possession of a
DOSTROVSKY A, SAGHER F. DIAGNOSTIC SIGNIFICANCE OF THE CULTURE METHOD IN CUTANEOUS LEISHMANIASIS (ORIENTAL SORE). Arch Derm Syphilol. 1946;54(5):543–551. doi:10.1001/archderm.1946.01510400049007
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