MANY years ago, Mitchell stated picturesquely that when there were failures in the culture of scrapings from the toes, the attempts should be repeated "not seven times, but seventy times seven." Still, most texts treat only superficially or not at all of the necessity of using discrimination and care when selecting cutaneous scrapings for microscopic examinations and of collecting and planting an abundance of material when the cultures are essayed. Doubtless some dermatologists are aware of this, but many of them do not practice it. In any event, it is a common experience in the Laboratory of Dermatological Research at the University of Pennsylvania to receive a dozen or less particles of scrapings, perhaps crushed and adherent between two glass slides which are bound together with rubber bands, with the request that both microscopic examination and culture be performed. This is not serious in the case of material from lesions
WEIDMAN FD, GLASS FA. "REPEAT" CULTURES IN DERMATOPHYTOSIS. Arch Derm Syphilol. 1948;57(3_PART_I):300–303. doi:10.1001/archderm.1948.01520150022003
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