THE METHOD of systematic observation in the hanging drop is widely used and has been universally accepted for detailed microscopic study of the morphologic structure and development of fungus cultures.
The technic of inoculation into the hanging drop is simple and well known: A drop of liquid medium (sugar broth or beer mash) is placed in the center of a cover slip under sterile conditions, and a small portion of the test culture is introduced into the thick of this drop; the culture must be grown in solid or liquid mediums in a test tube or in a big tube. The cover slip is next covered with a slide having a depression in its middle portion so that the center of the depression lies over the drop. The edges of the depression are smeared with petrolatum in order to fix the cover slip to the slide.
ZIMERINOV AA, RAFALOVICH SM. INVESTIGATION OF FUNGI IN HANGING DROP CULTURES: Preliminary Report. Arch Derm Syphilol. 1950;61(1):94–104. doi:10.1001/archderm.1950.01530080100010
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