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December 1952

CANDIDA ALBICANS: Rapid Identification in Pure Cultures with Carbon Dioxide on Modified Eosin-Methylene Blue Medium

Author Affiliations


From the Department of Pathology, College of Physicians and Surgeons, Columbia University.

AMA Arch Derm Syphilol. 1952;66(6):691-694. doi:10.1001/archderm.1952.01530310029003

RECENT reports1 of infections with Candida (Monilia) albicans following the use of antibiotics suggest the desirability of a simple, rapid, and reliable method for the differentiation of this fungus from the common closely related nonpathogenic species of the same genus. The procedure now in use requires considerable laboratory manipulation extending over several days. The method here described is a combination of simple techniques already familiar in clinical laboratories. It yields readily interpreted results in 18 hours. The fungus can be identified either in pure culture or in mixed cultures made directly from feces, mucous membranes, or skin. This paper describes only the results obtained with pure cultures. Use of the method with clinical specimens will be reported in another communication.

The culture medium is Levine eosin-methylene blue agar (difco®). To this is added a solution containing 10 mg. per cubic centimeter of

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