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May 1953

CANDIDA ALBICANSRapid Identification in Cultures Made Directly from Human Materials

Author Affiliations


From the Department of Pathology, College of Physicians and Surgeons, Columbia University.

AMA Arch Derm Syphilol. 1953;67(5):473-478. doi:10.1001/archderm.1953.01540050037008

IN A PREVIOUS paper1 it was reported that incubation of pure cultures made from thin saline suspensions on Levine eosin-methylene blue agar containing 0.1 mg. of aureomycin per cubic centimeter at 37 C. in 10% CO2 makes it possible to distinguish Candida albicans from other related species within 18 hours. In the present paper, a similarly rapid identification of this fungus in cultures made directly from samples of human clinical materials is described. The effects of incubating such cultures in air and in CO2 are compared.

The 114 specimens used in this study were made from feces, mouth and vaginal secretions, skin, and nail scrapings collected in the clinic from 54 patients with suspected moniliasis or undergoing treatment for or after recovery from proved moniliasis. In many instances materials for culture were taken from several areas of the same patient on the same day. Two samples were

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