The inhibitory effect of prophenpyridamine (Trimeton) maleate* (1-phenyl-1-[2-pyridyl] - 3-dimethylaminopropane maleate) on the growth of Blastomyces dermatitidis induced us to investigate certain aspects of this phenomenon. In our previous experiments † we noticed a partial inhibition of B. dermatitidis at 0.003 M prophenpyridamine maleate and complete suppression of growth at 0.015 M prophenpyridamine maleate.
1. Effect of Prophenpyridamine Maleate on Experimental Blastomycosis in Mice.— For investigation of the therapeutic implications of these findings, mice were infected with B. dermatitidis and treated with prophenpyridamine maleate. Twenty-eight albino mice of the Swiss strain (18 to 20 gm.) were infected by the intraperitoneal route with a heavy suspension of the yeast phase of B. dermatitidis suspended in 4% maltose, 1% peptone water. The organisms used for the mouse inoculations had been grown on blood agar in the incubator at 37 C